Determination of drugs of abuse in oral fluid using dried oral fluid spot assisted by 24-well plate and LC-MS/MS.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-05-01 Epub Date: 2025-05-22 DOI:10.1080/17576180.2025.2506348

Gabriela Ramos Borges, Bruno Pereira Dos Santos, Giovanna Cristiano de Gouveia, Carolina Silveira Dalanhol, Juliana Nichterwitz Scherer, Sarah Eller, Tiago Franco de Oliveira

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引用次数: 0

Abstract

Background: Oral fluid has proven to be an excellent matrix for drug testing. Several analytical techniques are available, including the use of dried spots, which have gained increasing recognition in recent years. In this study, a new method was developed and validated using dried oral fluid spot (DOFS) assisted by a 24-well plate for the simultaneous determination of thirteen drugs of abuse and metabolites in oral fluid by LC - MS/MS.

Methods: Samples were collected by expectoration and diluted with water. A 100 μL aliquot of sample was applied onto the spot, dried for 60 min, and extracted with 250 μL of acetonitrile:methanol (3:1). The method was validated according to the ANSI/ASB Standard 036 guideline, and 86 oral fluid samples were analyzed.

Results: The limits of quantification ranged from 1 to 5 ng/mL, and all calibration curves were linear. Precisions ranged from 2.0 to 13.0% and Bias fluctuated from -19.7 to 9.6%. Ionization suppression or enhancement was noted at -16.2 to 16.6%. Among the analyzed samples, 86% tested positive for at least one substance.

Conclusions: The findings confirm that the proposed method was successfully validated, providing a new bioanalytical approach for the detection of drugs of abuse in oral fluid.

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24孔板辅助干口服液斑法和LC-MS/MS法测定口服液中滥用药物。

背景：口服液已被证明是一种很好的药物试验基质。几种分析技术是可用的，包括使用干燥斑点，近年来已获得越来越多的认可。本研究建立了24孔板辅助干口服液斑点（DOFS）同时测定口服液中13种滥用药物及其代谢物的LC - MS/MS方法，并进行了验证。方法：用痰液法采集标本，用水稀释。取100 μL样品涂于现场，干燥60 min，用250 μL乙腈：甲醇（3:1）提取。根据ANSI/ASB标准036指南对该方法进行验证，并对86份口服液样品进行分析。结果：定量限范围为1 ~ 5 ng/mL，标定曲线线性良好。精密度范围为2.0 ~ 13.0%，偏差范围为-19.7 ~ 9.6%。电离抑制或增强在-16.2到16.6%之间。在分析的样本中，86%的样本至少有一种物质呈阳性。结论：该方法的有效性得到了验证，为口服液中滥用药物的检测提供了一种新的生物分析方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.