Average fluorescence intensity is affected by distance and penetration depth with near-infrared fluorescence imaging with indocyanine green in an ex vivo model.

IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES
Adrianna K Szostek, Janet A Grimes, Mandy L Wallace
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引用次数: 0

Abstract

Objective: The objective of this study was to evaluate the effect of indocyanine green (ICG) concentration, distance, angle, and penetration depth on average fluorescence intensity (AFI) when using 2 imaging devices in a canine ex vivo model.

Methods: 3 concentrations of ICG were evaluated (0.006, 0.011, and 0.022 mg/mL) for the effect of distance, penetration depth, angle, and ambient light on AFI. Penetration depth was assessed using serially stacked 1-mm sheets of a gelatin phantom. Each set of conditions was tested using a VITOM 3D and 30° 10-mm laparoscope. Polynomial regression was used to evaluate for significance.

Results: Average fluorescence intensity increased with increasing distance for the 30° 10-mm laparoscope. The VITOM 3D displayed higher AFIs at closer and farther distances. Average fluorescence intensity decreased as penetration depth increased for both devices. Ambient light had no effect on AFI, and AFI varied by angle, but statistical analysis for these variables was limited by insufficient data points.

Conclusions: Average fluorescence intensity was affected by distance, penetration depth, and angle for each ICG concentration and imaging device. There was no apparent effect on AFI with or without ambient light.

Clinical relevance: The interest in near-infrared fluorescence with ICG is growing in veterinary medicine. Optimization of protocols using these techniques can provide veterinarians with guidelines for the implementation of near-infrared fluorescence with ICG. Further studies are needed to assess the relationship between distance, depth, angle, and ambient light with AFI in vivo.

在离体模型中,吲哚菁绿近红外荧光成像的平均荧光强度受距离和穿透深度的影响。
目的:本研究的目的是在犬离体模型中,评价吲哚菁绿(ICG)浓度、距离、角度和穿透深度对2种成像装置平均荧光强度(AFI)的影响。方法:测定3种浓度(0.006、0.011、0.022 mg/mL)的ICG,观察距离、穿透深度、角度和环境光照对AFI的影响。通过连续堆叠1毫米的明胶模片来评估穿透深度。使用VITOM 3D和30°10-mm腹腔镜对每组条件进行测试。采用多项式回归进行显著性评价。结果:30°10mm腹腔镜平均荧光强度随距离增加而增加。VITOM 3D在更近和更远的距离上显示更高的afi。两种器件的平均荧光强度随穿透深度的增加而降低。环境光对AFI没有影响,AFI随角度变化,但由于数据点不足,这些变量的统计分析受到限制。结论:各ICG浓度和成像装置的距离、穿透深度和角度对平均荧光强度有影响。有无环境光对AFI均无明显影响。临床意义:兽医对近红外荧光与ICG的兴趣正在增长。使用这些技术优化方案可以为兽医提供近红外荧光ICG实施指南。需要进一步的研究来评估距离、深度、角度和环境光与体内AFI之间的关系。
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来源期刊
CiteScore
1.70
自引率
10.00%
发文量
186
审稿时长
3 months
期刊介绍: The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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