Utz H. Ermel, Harald Schwalbe, Alexey V. Cherepanov
{"title":"Cover Feature: Nanosecond Hyperquenching for Electron Cryo-Microscopy Without Air-Water Interface Artifacts","authors":"Utz H. Ermel, Harald Schwalbe, Alexey V. Cherepanov","doi":"10.1002/chem.202582903","DOIUrl":null,"url":null,"abstract":"<p><b>Partitioning of protein specimens</b> to the air–water interface (AWI) poses an acute problem in electron cryo-microscopy (ECM), causing a loss of biological function, subunit dissociation, protein denaturation and aggregation at the AWI with an insufficient number of visible molecular orientations. The outstanding method of ECM sampling–nanosecond hyperquenching (NHQ) solves this problem, yielding vitrified nanofilms without AWI-bound protein. The Research Article by U. H. Ermel, H. Schwalbe and A. V. Cherepanov (DOI: 10.1002/chem.202403878) offers a detailed characterization of protein distribution in vitrified nanofilms and the mechanism of protein exclusion from AWI in terms of macroscopic shock-wave mechanics.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":144,"journal":{"name":"Chemistry - A European Journal","volume":"31 29","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/chem.202582903","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry - A European Journal","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/chem.202582903","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Partitioning of protein specimens to the air–water interface (AWI) poses an acute problem in electron cryo-microscopy (ECM), causing a loss of biological function, subunit dissociation, protein denaturation and aggregation at the AWI with an insufficient number of visible molecular orientations. The outstanding method of ECM sampling–nanosecond hyperquenching (NHQ) solves this problem, yielding vitrified nanofilms without AWI-bound protein. The Research Article by U. H. Ermel, H. Schwalbe and A. V. Cherepanov (DOI: 10.1002/chem.202403878) offers a detailed characterization of protein distribution in vitrified nanofilms and the mechanism of protein exclusion from AWI in terms of macroscopic shock-wave mechanics.
在电子冷冻显微镜（ECM）中，蛋白质样品向空气-水界面（AWI）的分配是一个严重的问题，导致生物功能丧失，亚基解离，蛋白质变性和AWI聚集，可见分子取向数量不足。ECM的一种杰出的采样方法——纳秒超淬火（NHQ）解决了这一问题，得到了不含awi结合蛋白的玻璃化纳米膜。这篇由U. H. Ermel， H. Schwalbe和a . V. Cherepanov撰写的研究文章（DOI: 10.1002/chem.202403878）提供了玻璃化纳米膜中蛋白质分布的详细特征，以及从宏观冲击波力学角度排除AWI的蛋白质机制。
期刊介绍：
Chemistry—A European Journal is a truly international journal with top quality contributions (2018 ISI Impact Factor: 5.16). It publishes a wide range of outstanding Reviews, Minireviews, Concepts, Full Papers, and Communications from all areas of chemistry and related fields.
Based in Europe Chemistry—A European Journal provides an excellent platform for increasing the visibility of European chemistry as well as for featuring the best research from authors from around the world.
All manuscripts are peer-reviewed, and electronic processing ensures accurate reproduction of text and data, plus short publication times.
The Concepts section provides nonspecialist readers with a useful conceptual guide to unfamiliar areas and experts with new angles on familiar problems.
Chemistry—A European Journal is published on behalf of ChemPubSoc Europe, a group of 16 national chemical societies from within Europe, and supported by the Asian Chemical Editorial Societies. The ChemPubSoc Europe family comprises: Angewandte Chemie, Chemistry—A European Journal, European Journal of Organic Chemistry, European Journal of Inorganic Chemistry, ChemPhysChem, ChemBioChem, ChemMedChem, ChemCatChem, ChemSusChem, ChemPlusChem, ChemElectroChem, and ChemistryOpen.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
