Assessment of chemical composition and in vitro and in silico anticarcinogenic activity of bee bread samples from Eastern Anatolia (Kars)

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

European Food Research and Technology Pub Date : 2025-02-13 DOI:10.1007/s00217-025-04679-0

Güven Gülbaz, Giray Buğra Akbaba, Füreya Elif Öztürkkan

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Abstract

In this study, aqueous extracts of bee bread samples collected from five different regions in Eastern Anatolia (Kars, Turkey) were obtained by ultrasonic wave-assisted extraction method. Moisture, ash, acidity and pH values of bee bread samples were measured. Phenolic components of aqueous extracts were determined by LC-HRMS method. The cytotoxicity of these extracts on colon (DLD-1), breast (MCF-7), cervix (HeLa) and prostate cancer (PC-3) cells was determined by the MTT method. The interactions of genistein, naringenin and vitamin C, which are major components of the extracts, with important cancer targets such as human thymidylate synthase, c-MYC transcription factor, cyclin-dependent kinase and androgen receptor prostate cancer targets were investigated by Molecular Docking method. According to the findings, all extracts caused higher cytotoxicity on MCF-7 cancer cells compared to other cell lines, while they caused low cytotoxicity on PC-3 cells. Molecular Docking results show that the naringenin compound, whose anticarcinogenic effects have been supported by previous studies, may be a successful agent in inhibiting the active sites of selected cancer cell targets. When all these results are evaluated, it is recommended that the antitumoral effect of bee bread for the treatment of cancer disease be supported by further studies.

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东安纳托利亚（卡尔斯）蜜蜂面包样品的化学成分及体外和体内抗癌活性评价

本研究采用超声波辅助提取法，从土耳其东部安纳托利亚（Kars, Turkey）五个不同地区的蜜蜂面包样品中提取水提物。测定了蜜蜂面包样品的水分、灰分、酸度和pH值。采用LC-HRMS法测定水提液中酚类成分。MTT法测定各提取物对结肠癌（DLD-1）、乳腺癌（MCF-7）、宫颈癌（HeLa）和前列腺癌（PC-3）细胞的细胞毒性。采用分子对接法研究了黄酮提取物的主要成分染料木素、柚皮素和维生素C与人胸腺苷酸合成酶、C - myc转录因子、细胞周期蛋白依赖性激酶和雄激素受体前列腺癌靶点等重要肿瘤靶点的相互作用。根据研究结果，与其他细胞系相比，所有提取物对MCF-7癌细胞的细胞毒性更高，而对PC-3细胞的细胞毒性较低。分子对接结果表明，柚皮素化合物可能是一种成功的抑制肿瘤细胞靶点活性位点的药物，其抗癌作用已得到前期研究的支持。当所有这些结果被评估后，我们建议蜜蜂面包治疗癌症的抗肿瘤作用需要进一步的研究来支持。

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来源期刊

European Food Research and Technology 工程技术-食品科技

CiteScore

6.60

自引率

3.00%

发文量

232

审稿时长

2.0 months

期刊介绍： The journal European Food Research and Technology publishes state-of-the-art research papers and review articles on fundamental and applied food research. The journal''s mission is the fast publication of high quality papers on front-line research, newest techniques and on developing trends in the following sections: -chemistry and biochemistry- technology and molecular biotechnology- nutritional chemistry and toxicology- analytical and sensory methodologies- food physics. Out of the scope of the journal are: - contributions which are not of international interest or do not have a substantial impact on food sciences, - submissions which comprise merely data collections, based on the use of routine analytical or bacteriological methods, - contributions reporting biological or functional effects without profound chemical and/or physical structure characterization of the compound(s) under research.