Characterization of homogeneous energy transfer kinetics by time-correlated single photon counting

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2025-05-21 DOI:10.1016/j.jphotochem.2025.116514

Yongjia Wang, Di Tian, Zece Zhu

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引用次数: 0

Abstract

Energy transfer between identical molecules is difficult to measure since the donors and acceptors in this homogeneous energy transfer emit fluorescence with the same or similar wavelength. Herein, a time-correlated single photon counting system was developed to measure the fluorescence kinetics with a temporal resolution of sub-nanoseconds for analyzing the rate of homogeneous energy transfers. By studying the fluorescence kinetic curves of Rhodamine 6G in different solvents and films, it was found that the average energy transfer rate decreases with increasing concentration, indicating that multiple energy transfers at higher concentrations lead to an extended duration of the excited state. Compared with transient absorption spectroscopy, this method can effortlessly measure samples with a wide concentration range at a lower cost. Furthermore, it can be extended to investigate other excited-state dynamics such as intersystem crossing and triplet-state energy transfer, providing a novel analytical tool for advanced photophysical research.

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用时间相关单光子计数表征均匀能量传递动力学

相同分子之间的能量转移很难测量，因为在这种均匀的能量转移中，供体和受体发出的荧光具有相同或相似的波长。在此，开发了一个时间相关的单光子计数系统来测量荧光动力学，时间分辨率为亚纳秒，用于分析均匀能量转移率。通过对罗丹明6G在不同溶剂和薄膜中的荧光动力学曲线的研究，发现平均能量传递速率随浓度的增加而降低，说明高浓度下的多次能量传递导致激发态持续时间延长。与瞬态吸收光谱法相比，该方法测量样品的浓度范围广，测量成本低。此外，它还可以扩展到其他激发态动力学的研究，如系统间交叉和三态能量转移，为先进的光物理研究提供了一种新的分析工具。

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.