Post-weaning exposure to cannabidiol disrupts testicular cytoarchitecture and sperm quality in mice

IF 3.3 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2025-05-20 DOI:10.1016/j.reprotox.2025.108952

Renata K. Carvalho , Maingredy R. Souza , Akemy N. Nishimura , Edvaldo M. Silva , Cinthia R.B. Silva , Francisco S. Guimarães , Monica L. Andersen , Simone M.T. Sabóia-Morais , Renata Mazaro-Costa

{"title":"Post-weaning exposure to cannabidiol disrupts testicular cytoarchitecture and sperm quality in mice","authors":"Renata K. Carvalho , Maingredy R. Souza , Akemy N. Nishimura , Edvaldo M. Silva , Cinthia R.B. Silva , Francisco S. Guimarães , Monica L. Andersen , Simone M.T. Sabóia-Morais , Renata Mazaro-Costa","doi":"10.1016/j.reprotox.2025.108952","DOIUrl":null,"url":null,"abstract":"<div><div>Cannabidiol (CBD) is a natural cannabinoid with a wide range of potential therapeutic applications, including as an anticonvulsant and for the treatment of inflammatory conditions. It is known that CBD interacts with the endocannabinoid system, which plays a crucial role in various physiological functions. However, its effects on male reproduction have not yet been fully elucidated. Thus, the aim of this study was to evaluate the <em>in vivo</em> effects of CBD on testicular cytoarchitecture and sperm quality in mice. Twenty-one-day old male Swiss mice received intragastric doses of CBD (15 or 30 mg/kg/day) for 34 consecutive days. A control group received sunflower oil. Both doses of CBD reduced the number of Sertoli cells at stages VII–VIII, IX and XII of spermatogenesis. A significant decrease in proliferating cell nuclear antigen (PCNA)-positive spermatocytes at stages VII–VIII was observed in the CBD15 group. In the interstitial compartment of the testis, no significant differences were found in the diameter and volume of Leydig cell nuclei or in the immunostaining of these cells for PCNA in the control and CBD-treated groups. Both doses of CBD reduced the percentage of viable spermatozoa and the percentage of morphologically normal spermatozoa. These findings suggest that daily exposure to CBD may reduce sperm quality, and the mechanisms responsible may be related to perturbations in the endocannabinoid system during spermatogenesis.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"135 ","pages":"Article 108952"},"PeriodicalIF":3.3000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive toxicology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0890623825001236","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Cannabidiol (CBD) is a natural cannabinoid with a wide range of potential therapeutic applications, including as an anticonvulsant and for the treatment of inflammatory conditions. It is known that CBD interacts with the endocannabinoid system, which plays a crucial role in various physiological functions. However, its effects on male reproduction have not yet been fully elucidated. Thus, the aim of this study was to evaluate the in vivo effects of CBD on testicular cytoarchitecture and sperm quality in mice. Twenty-one-day old male Swiss mice received intragastric doses of CBD (15 or 30 mg/kg/day) for 34 consecutive days. A control group received sunflower oil. Both doses of CBD reduced the number of Sertoli cells at stages VII–VIII, IX and XII of spermatogenesis. A significant decrease in proliferating cell nuclear antigen (PCNA)-positive spermatocytes at stages VII–VIII was observed in the CBD15 group. In the interstitial compartment of the testis, no significant differences were found in the diameter and volume of Leydig cell nuclei or in the immunostaining of these cells for PCNA in the control and CBD-treated groups. Both doses of CBD reduced the percentage of viable spermatozoa and the percentage of morphologically normal spermatozoa. These findings suggest that daily exposure to CBD may reduce sperm quality, and the mechanisms responsible may be related to perturbations in the endocannabinoid system during spermatogenesis.

查看原文本刊更多论文

断奶后暴露于大麻二酚会破坏小鼠睾丸细胞结构和精子质量

大麻二酚（CBD）是一种天然大麻素，具有广泛的潜在治疗应用，包括抗惊厥药和治疗炎症。众所周知，CBD与内源性大麻素系统相互作用，在各种生理功能中起着至关重要的作用。然而，它对男性生殖的影响尚未完全阐明。因此，本研究的目的是评估CBD对小鼠睾丸细胞结构和精子质量的体内影响。21日龄雄性瑞士小鼠连续34天灌胃CBD（15或30 mg/kg/天）。对照组服用葵花籽油。两种剂量的CBD在精子发生的第VII-VIII、IX和XII阶段都减少了支持细胞的数量。CBD15组在第7 - 8期观察到增殖细胞核抗原（PCNA）阳性精母细胞显著减少。在睾丸间质室中，对照组和cbd处理组间质间质细胞核的直径和体积以及这些细胞对PCNA的免疫染色均无显著差异。两种剂量的CBD都降低了存活精子的百分比和形态正常精子的百分比。这些发现表明，每天暴露于CBD可能会降低精子质量，其机制可能与精子发生过程中内源性大麻素系统的扰动有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.