Comprehensive transcriptomic and proteomic analysis reveals biological changes and potential regulatory mechanisms of endothelial cells under heat stress conditions

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology Pub Date : 2025-05-01 DOI:10.1016/j.jtherbio.2025.104138

Keran Jia , Jie Zheng , Saicong Hu , Daxin Lei , Mengyu Jiang , Jiajia Zhang , Mengge Cui , Jia Wang , Yan Cui , Fukun Wang

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Abstract

Endothelial cells are crucial for vascular function, and they respond to heat stress by altering gene and protein expression. This study investigated the impact of heat stress on endothelial cells using transcriptomic and proteomic analyses. Human umbilical vein endothelial cells (HUVECs) were exposed to heat stress at 43 °C for 6 h, resulting in significant changes in gene and protein expression patterns. In particular, ORAI1, a key regulator of intracellular Ca²⁺ levels, was significantly upregulated. The integration of transcriptomic and proteomic data revealed genes and proteins associated with endothelial cell damage and repair processes. Validation experiments confirmed the upregulation of ORAI1 and increased intracellular Ca²⁺ levels under heat stress. These findings enhance our understanding of endothelial cell responses to heat stress and suggest potential therapeutic targets for heat-related diseases.

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综合转录组学和蛋白质组学分析揭示了热应激条件下内皮细胞的生物学变化和潜在的调节机制

内皮细胞对血管功能至关重要，它们通过改变基因和蛋白质表达来应对热应激。本研究利用转录组学和蛋白质组学分析研究了热应激对内皮细胞的影响。人脐静脉内皮细胞（HUVECs）在43°C下暴露于热应激6小时，导致基因和蛋白质表达模式发生显著变化。特别是，细胞内Ca2+水平的关键调节因子ORAI1显著上调。转录组学和蛋白质组学数据的整合揭示了与内皮细胞损伤和修复过程相关的基因和蛋白质。验证实验证实，在热应激下，ORAI1表达上调，细胞内Ca2+水平升高。这些发现增强了我们对内皮细胞对热应激反应的理解，并提出了热相关疾病的潜在治疗靶点。

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来源期刊

Journal of thermal biology 生物-动物学

CiteScore

5.30

自引率

7.40%

发文量

196

审稿时长

14.5 weeks

期刊介绍： The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles