Biocontrol potential of Arabidopsis thaliana against Biomphalaria glabrata, the intermediate host of Schistosoma mansoni, focusing on biological, physiological, histopathological, and genotoxic impacts

IF 2 Q3 INFECTIOUS DISEASES

Parasite Epidemiology and Control Pub Date : 2025-05-12 DOI:10.1016/j.parepi.2025.e00428

Najla Y. Beit Elmal , Fathy A. Abdel Ghaffar , Salwa A.H. Hamdi , Amina M. Ibrahim , Mona F. Fol , Nesma A. Mostafa

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引用次数: 0

Abstract

Controlling Biomphalaria species with plant products is crucial in the treatment of schistosomiasis since it is eco-friendly and generally less harmful to non-target organisms. The goal of this study was to determine the molluscicidal activity of an aqueous extract of Arabidopsis thaliana leaves against Biomphalaria glabrata. A preliminary phytochemical screening test revealed the presence of flavonoids, terpenoids, steroids, anthraquinones, alkaloids, saponins, tannins, and carbohydrates. Following exposure to sub-lethal concentrations (LC₁₀ 75.62 mg/L and LC₂₅ 90.52 mg/L) of aqueous extract of A. thaliana, a reduction in survival, reproductive, and fecundity rates of B. glabrata were detected, as well as a substantial decrease in GSH, CAT, and SOD, while increasing MDA and NO levels. In addition, there was an increase in liver and renal functions as well as lipid profiles, compared to the control group. Histopathological examination of the digestive gland of treated B. glabrata showed a shrinkage of the tubules and an increase in the inter-tubular spaces as well as degeneration in the oocytes and spermatocytes of the hermaphrodite glands with a loss of connective tissues between the acini. Also, the comet assay revealed a genotoxic effect of aqueous extract of A. thaliana on B. glabrata, with a significant increase in the tail moment, tail length, and DNA percentage reflecting DNA damage compared to the control group. Conclusively, Arabidopsis thaliana aqueous extract acts as a natural molluscicidal agent against Biomphalaria glabrata.

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拟南芥对曼氏血吸虫中间寄主光光生物phalaria glabrata的生物防治潜力，主要包括生物学、生理学、组织病理学和基因毒性影响

用植物产品控制生物phalaria物种在血吸虫病治疗中至关重要，因为它是生态友好的，通常对非目标生物的危害较小。本研究的目的是测定拟南芥叶水提物对光斑生物的杀螺活性。初步的植物化学筛选试验显示黄酮类、萜类、类固醇、蒽醌类、生物碱、皂苷、单宁和碳水化合物。暴露于亚致死浓度（LC10 75.62 mg/L和LC25 90.52 mg/L）的拟南草水提物后，发现光斑拟南草的存活率、繁殖率和繁殖率下降，GSH、CAT和SOD显著降低，MDA和NO水平升高。此外，与对照组相比，肝脏和肾脏功能以及血脂都有所增加。对处理过的光斑扁豆消化腺的组织病理学检查显示小管缩小，管间间隙增加，雌雄同体腺体的卵母细胞和精母细胞变性，腺泡之间的结缔组织缺失。此外，彗星试验显示，拟南芥水提取物对光斑拟南芥具有遗传毒性作用，与对照组相比，尾巴力矩、尾巴长度和反映DNA损伤的DNA百分比显著增加。综上所述，拟南芥水提物是一种天然的杀螺剂。

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来源期刊

Parasite Epidemiology and Control Medicine-Infectious Diseases

CiteScore

5.70

自引率

3.10%

发文量

审稿时长

17 weeks

期刊介绍： Parasite Epidemiology and Control is an Open Access journal. There is an increasing amount of research in the parasitology area that analyses the patterns, causes, and effects of health and disease conditions in defined populations. This epidemiology of parasite infectious diseases is predominantly studied in human populations but also spans other major hosts of parasitic infections and as such this journal will have a broad remit. We will focus on the major areas of epidemiological study including disease etiology, disease surveillance, drug resistance and geographical spread and screening, biomonitoring, and comparisons of treatment effects in clinical trials for both human and other animals. We will also look at the epidemiology and control of vector insects. The journal will also cover the use of geographic information systems (Epi-GIS) for epidemiological surveillance which is a rapidly growing area of research in infectious diseases. Molecular epidemiological approaches are also particularly encouraged.