Ecological risks under combined pollution: Toxicological effects of clothianidin and microplastics on Penaeus vannamei

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-05-19 DOI:10.1016/j.aquatox.2025.107417

Zhi Luo , Zhi-Yu Lin , Zhen-Fei Li , Zhen-Qiang Fu , Feng-Lu Han , Er-Chao Li

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引用次数: 0

Abstract

In real-world conditions, multiple pollutants coexist, making it insufficient to study a single pollutant's effects. Clothianidin, a widely present neonicotinoid insecticide, poses significant ecological risks due to its unique mechanism of action. Similarly, microplastics, another prevalent pollutant, have notable ecological impacts. This study aims to assess the ecotoxicological effects of clothianidin and microplastics on P. vannamei over 28 days, both individually and in combination. The study found that combined exposure significantly inhibited the shrimp's weight gain rate and hepatosomatic index, and significant changes were observed in the shrimp's immune defense, signal transmission, and energy metabolism. Key findings include changes in levels of hemocyanin, respiratory burst, nitric oxide, and phenol oxidase, as well as disturbances in enzyme activities of Lactate dehydrogenase, NADPH-dependent isocitrate dehydrogenase, and Succinate dehydrogenase. Additionally, fluctuations in levels of acetylcholine, dopamine, and acetylcholinesterase confirmed disruptions in neural signaling. Transcriptomic analysis further revealed the profound impact of these pollutants on gene expression and metabolic processes in the hepatopancreas and nervous system. This comprehensive assessment highlights the potential impact on shrimp growth and emphasizes the ecological risks of clothianidin and microplastics, providing insights for future risk assessments and the identification of biomarkers.

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复合污染下的生态风险：噻虫胺和微塑料对凡纳滨对虾的毒理学效应

在现实条件下，多种污染物共存，研究单一污染物的影响是不够的。噻虫胺是一种广泛存在的新烟碱类杀虫剂，因其独特的作用机制而具有重大的生态风险。同样，微塑料，另一种普遍的污染物，也有显著的生态影响。本研究旨在评价噻虫胺和微塑料单独或联合使用对凡纳滨拟虫28 d的生态毒理学效应。研究发现，联合暴露显著抑制了对虾的增重率和肝体指数，对虾的免疫防御、信号传递和能量代谢发生了显著变化。主要发现包括血青素、呼吸爆发、一氧化氮和酚氧化酶水平的变化，以及乳酸脱氢酶、nadph依赖性异柠檬酸脱氢酶和琥珀酸脱氢酶活性的紊乱。此外，乙酰胆碱、多巴胺和乙酰胆碱酯酶水平的波动证实了神经信号的中断。转录组学分析进一步揭示了这些污染物对肝胰腺和神经系统基因表达和代谢过程的深远影响。这项综合评估强调了对虾生长的潜在影响，并强调了噻虫胺和微塑料的生态风险，为未来的风险评估和生物标志物的鉴定提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.