Colorimetric Optode Sensor with Tripodal Ionophore for Rapid Urinary Ammonium Determination

IF 8.2 1区化学 Q1 CHEMISTRY, ANALYTICAL

ACS Sensors Pub Date : 2025-05-14 DOI:10.1021/acssensors.5c0075610.1021/acssensors.5c00756

Fangmei Fu, XinYu Zhang, Wei Wang and Xiaojiang Xie*,

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Abstract

The amount of ammonium excreted in urine is a crucial indicator for assessing renal metabolic acidosis. Therefore, there is a significant demand for chemical sensors capable of accurately determining urinary ammonium concentrations with high throughput. In this study, we developed an ammonia chemical sensor based on the ion-selective optode principle. This sensor employs strategically designed ionophores and a PTFE gas-permeable film to enhance selectivity for free NH₃. The low basicity of the chromoionophore indicator and the weak affinity of the tripodal ammonium receptor (L, log β = 4.62 ± 0.18) contributed to a markedly reduced response time, achieving detection within seconds, a significant improvement over previous sensors. A limit of detection (LOD) of approximately 15 nM was obtained in colorimetric mode. Total inorganic ammonia content in undiluted human urine was successfully determined using the sensor. Additionally, the optode was adapted into a multiwell format, offering potential for high-throughput point-of-care testing of urinary ammonium concentrations and other samples.

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三脚架离子载体比色光电传感器快速测定尿铵

尿铵排泄量是评估肾代谢性酸中毒的重要指标。因此，对能够高通量准确测定尿铵浓度的化学传感器有很大的需求。在这项研究中，我们开发了一种基于离子选择光电原理的氨化学传感器。该传感器采用精心设计的离子载体和聚四氟乙烯透气膜，以提高对游离NH3的选择性。色离子载体指示剂的低碱度和三足铵受体的弱亲和力（L, log β = 4.62±0.18）显著缩短了响应时间，实现了几秒内的检测，比以前的传感器有了显著的改进。在比色模式下，检测限（LOD）约为15 nM。利用该传感器成功地测定了未稀释人体尿液中总无机氨含量。此外，该光电二极管可用于多孔格式，为尿铵浓度和其他样品的高通量即时检测提供了潜力。

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来源期刊

ACS Sensors Chemical Engineering-Bioengineering

CiteScore

14.50

自引率

3.40%

发文量

372

期刊介绍： ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.