Clinicopathological insights and prognostic implications of DEK in association with apoptosis-regulating factors in ovarian cancer.

IF 2.8 3区医学 Q2 ONCOLOGY

Clinical & Translational Oncology Pub Date : 2025-05-20 DOI:10.1007/s12094-025-03944-8

Trisha Choudhury, Ranita Pal, Madhurima Ghosh, Sriparna Chatterjee, Sinjini Sarkar, Manisha Vernekar, Partha Nath, Vilas D Nasare

{"title":"Clinicopathological insights and prognostic implications of DEK in association with apoptosis-regulating factors in ovarian cancer.","authors":"Trisha Choudhury, Ranita Pal, Madhurima Ghosh, Sriparna Chatterjee, Sinjini Sarkar, Manisha Vernekar, Partha Nath, Vilas D Nasare","doi":"10.1007/s12094-025-03944-8","DOIUrl":null,"url":null,"abstract":"Purpose: Ovarian cancer is one of the most common gynecologic malignancies of the present era. Dysregulation of apoptosis is considered as one of the most important factors for malignant transformation. DEK is a ubiquitous protein, and its downregulation induces apoptosis by altering BCL-2, BAX, and CASPASE-3 expressions. This study illuminates the cumulative clinical usefulness of DEK and related apoptotic proteins.Methods: A total of 119 patients were enrolled during 2021-2023. Demographic and clinicopathological data were recorded at presentation, and the follow-up was done till August 2024. Tissue samples were analyzed using immunohistochemistry and real-time PCR. A paired t test assessed gene expression between normal and malignant tissues of different treatment strategies. The crosstab was performed to find the association of DEK, BCL-2, BAX, and CASPASE-3 with clinicopathological features. Pearson's correlation was used to predict the association of DEK with other apoptotic factors. Survival and hazard risk were evaluated using log-rank and Cox regression.Results: The mean age of OC patients was 47.61 ± 12.5 years, presented with advanced stage (90.7%) and grade (85.3%). Most of them were post-menopausal (68.08%) and had unhygienic (76.5%) regular menstrual cycles (89.1%), and also experienced early pregnancy (61.8%). Some of these factors are related to a hazard risk (HR > 1). DEK and apoptotic proteins were upregulated in OC than in normal (p ≤ 0.01). DEK was positively correlated with BCL-2, BAX, and CASPASE-3, at both mRNA and protein levels, and only BAX showed significance in both (p ≤ 0.05). All the selected genes are independent risk factors for survival of OC (HR > 1), but only DEK and CASPASE-3 were significantly associated with poor survival (p ≤ 0.05).Conclusions: Dysregulation of DEK, CASPASE-3, and BAX/BCL-2 is associated with poor overall survival. Further, this study highlights the correlation between DEK and key apoptotic regulators, emphasizing the critical role of DEK in OC prognosis.","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical & Translational Oncology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12094-025-03944-8","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ONCOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: Ovarian cancer is one of the most common gynecologic malignancies of the present era. Dysregulation of apoptosis is considered as one of the most important factors for malignant transformation. DEK is a ubiquitous protein, and its downregulation induces apoptosis by altering BCL-2, BAX, and CASPASE-3 expressions. This study illuminates the cumulative clinical usefulness of DEK and related apoptotic proteins.

Methods: A total of 119 patients were enrolled during 2021-2023. Demographic and clinicopathological data were recorded at presentation, and the follow-up was done till August 2024. Tissue samples were analyzed using immunohistochemistry and real-time PCR. A paired t test assessed gene expression between normal and malignant tissues of different treatment strategies. The crosstab was performed to find the association of DEK, BCL-2, BAX, and CASPASE-3 with clinicopathological features. Pearson's correlation was used to predict the association of DEK with other apoptotic factors. Survival and hazard risk were evaluated using log-rank and Cox regression.

Results: The mean age of OC patients was 47.61 ± 12.5 years, presented with advanced stage (90.7%) and grade (85.3%). Most of them were post-menopausal (68.08%) and had unhygienic (76.5%) regular menstrual cycles (89.1%), and also experienced early pregnancy (61.8%). Some of these factors are related to a hazard risk (HR > 1). DEK and apoptotic proteins were upregulated in OC than in normal (p ≤ 0.01). DEK was positively correlated with BCL-2, BAX, and CASPASE-3, at both mRNA and protein levels, and only BAX showed significance in both (p ≤ 0.05). All the selected genes are independent risk factors for survival of OC (HR > 1), but only DEK and CASPASE-3 were significantly associated with poor survival (p ≤ 0.05).

Conclusions: Dysregulation of DEK, CASPASE-3, and BAX/BCL-2 is associated with poor overall survival. Further, this study highlights the correlation between DEK and key apoptotic regulators, emphasizing the critical role of DEK in OC prognosis.

查看原文本刊更多论文

卵巢癌中DEK与凋亡调节因子相关的临床病理观察和预后意义。

目的：卵巢癌是当今最常见的妇科恶性肿瘤之一。细胞凋亡失调被认为是恶性转化的重要因素之一。DEK是一种普遍存在的蛋白，其下调可通过改变BCL-2、BAX和CASPASE-3的表达诱导细胞凋亡。本研究阐明了DEK及相关凋亡蛋白的累积临床应用价值。方法：2021-2023年共纳入119例患者。入院时记录人口学和临床病理资料，随访至2024年8月。采用免疫组织化学和实时荧光定量PCR对组织样本进行分析。配对t检验评估不同治疗策略的正常组织和恶性组织之间的基因表达。采用交叉表法寻找DEK、BCL-2、BAX和CASPASE-3与临床病理特征的关系。采用Pearson相关法预测DEK与其他凋亡因子的相关性。采用log-rank和Cox回归评估生存和危害风险。结果：OC患者平均年龄为47.61±12.5岁，分为晚期（90.7%）和分级（85.3%）。其中以绝经后（68.08%）、月经周期不卫生（76.5%）、规律（89.1%）和早孕（61.8%）居多。其中一些因素与危害风险有关（HR bbb1）。OC组DEK和凋亡蛋白表达明显高于正常组（p≤0.01）。DEK与BCL-2、BAX、CASPASE-3在mRNA和蛋白水平上均呈正相关，仅BAX与BCL-2、BAX均有显著性差异（p≤0.05）。所选基因均为OC生存的独立危险因素（HR >1），只有DEK和CASPASE-3与OC生存不良有显著相关（p≤0.05）。结论：DEK、CASPASE-3和BAX/BCL-2的失调与总生存期差有关。进一步，本研究强调了DEK与关键凋亡调节因子之间的相关性，强调了DEK在OC预后中的关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinical & Translational Oncology 医学-肿瘤学

CiteScore

6.20

自引率

2.90%

发文量

240

审稿时长

1 months

期刊介绍： Clinical and Translational Oncology is an international journal devoted to fostering interaction between experimental and clinical oncology. It covers all aspects of research on cancer, from the more basic discoveries dealing with both cell and molecular biology of tumour cells, to the most advanced clinical assays of conventional and new drugs. In addition, the journal has a strong commitment to facilitating the transfer of knowledge from the basic laboratory to the clinical practice, with the publication of educational series devoted to closing the gap between molecular and clinical oncologists. Molecular biology of tumours, identification of new targets for cancer therapy, and new technologies for research and treatment of cancer are the major themes covered by the educational series. Full research articles on a broad spectrum of subjects, including the molecular and cellular bases of disease, aetiology, pathophysiology, pathology, epidemiology, clinical features, and the diagnosis, prognosis and treatment of cancer, will be considered for publication.