Integrating N-glycan and CODEX imaging reveal cell-specific protein glycosylation in healthy human lung†

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2025-05-20 DOI:10.1039/D4MO00230J

Dušan Veličković, Jeffrey Purkerson, Harsh Bhotika, Heidie Huyck, Geremy Clair, Gloria S. Pryhuber and Christopher Anderton

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Abstract

Identifying cell-specific glycan structures in human lungs is critical for understanding the chemistry and mechanisms that guide cell–cell and cell–matrix interactions and determining nuanced functions of specific glycosylation. Our dual-modality omics platform, which uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to profile glycan chemistry at 50 μm × 50 μm scale, combined with co-detection by indexing (CODEX) to provide cell identification from the exact same tissue section, is a significant step in this direction. It enabled us to detect, differentiate, and reveal chemical properties of N-glycans in the various cell types of a human lung, suggesting the cell-specific function of distinct carbohydrate moieties. This innovative technological combination bridges the gap between the specific protein glycosylation and their cellular origin, paving the way for targeted studies in the lungs and many other human tissues where glycans mediate cell–cell recognition events.

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整合n -聚糖和CODEX成像揭示健康人肺细胞特异性蛋白糖基化。

鉴定人类肺部细胞特异性糖基结构对于理解指导细胞-细胞和细胞-基质相互作用的化学和机制以及确定特异性糖基化的细微功能至关重要。我们的双模组学平台是朝着这个方向迈出的重要一步，该平台使用基质辅助激光解吸/电离（MALDI）质谱成像（MSI）在50 μm × 50 μm尺度上分析聚糖化学，结合索引共同检测（CODEX）从完全相同的组织切片提供细胞鉴定。它使我们能够检测、区分和揭示人类肺各种细胞类型中n -聚糖的化学性质，提示不同碳水化合物部分的细胞特异性功能。这种创新的技术组合弥合了特定蛋白质糖基化与其细胞起源之间的差距，为在肺部和许多其他人体组织中进行靶向研究铺平了道路，其中聚糖介导细胞-细胞识别事件。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.