M P Smuts, P R de Bruin, G T Fosgate, L Vorster, M de la Rey, C Lichtenberg, C Young, B S Durrant, L C Meyer, D E Holm
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引用次数: 0
Abstract
Background: Population numbers of free-roaming rhinoceros are dwindling while natural breeding in captive populations is challenged by sub-optimal fertility. When natural breeding programmes fail and population numbers continue to decline, the use of assisted reproduction technologies (ART) as a conservation tool to save wildlife species from extinction is imperative. Assisted breeding programmes in rhinoceros are challenging and attempts to develop species-specific ART beyond artificial insemination have been limited. This study evaluates various ex- and in-situ factors that could potentially affect in vitro oocyte maturation (IVM) in the Southern white rhinoceros (SWR).
Objective: To identify predictors of oocyte maturation following ovum pick-up (OPU) procedures performed on a large number of rhinoceros under different management conditions.
Methods: Data from 85 OPU procedures between November 2018 and June 2023 were obtained for this study. A total of 23 factors (three animal, four environmental, 16 laboratory) were evaluated as potentially affecting IVM success.
Results: Of the 85 procedures performed on 59 SWR, a total of 265 oocytes were collected with a median of two oocytes (range 0-18) per OPU. Eighteen (18) of the procedures yielded 0 oocytes. The overall maturation rate was 30% (80/265), and six identified factors had significant univariate associations with maturation outcome, of which three remained in the multivariable model.
Conclusion: Farming system, season and oocyte density in maturation medium (> 100 ul/oocyte) were independently associated with improved maturation success (p < 0.05).
期刊介绍：
The Journal of the South African Veterinary Association is a contemporary multi-disciplinary scientific mouthpiece for Veterinary Science in South Africa and abroad. It provides veterinarians in South Africa and elsewhere in the world with current scientific information across the full spectrum of veterinary science. Its content therefore includes reviews on various topics, clinical and non-clinical articles, research articles and short communications as well as case reports and letters.
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