{"title":"254 nm Ultraviolet C Light Emitting Diode (LED) Radiation Enhances Wound Healing in a Rat Model of Full-Thickness Skin Defects.","authors":"Wei Chen, Zhao Zhang, Xiao Zhang, Jing Zuo, Xiao Li, Shuang-Cheng Li, Yue-Ming Gao","doi":"10.1002/lsm.70030","DOIUrl":null,"url":null,"abstract":"<p><strong>Significance: </strong>254 nm short wave ultraviolet (UVC) emitted by low-pressure mercury lamp is effective in the treatment of skin wounds. With the emergence of deep ultraviolet light-emitting diodes (LED), it is expected to significantly expand the clinical indications of UVC. However, whether 254 nm UVC generated by LED light source can promote skin wound healing has not been reported.</p><p><strong>Aim: </strong>To clarify the effect of 254 nm UVC-LED light source on promoting skin wound healing and explore the mechanism of UVC-LED promoting wound healing.</p><p><strong>Approach: </strong>The full-thickness wound model of rat back skin was constructed, and the skin wounds of rats were treated with low-pressure mercury lamp and 254 nm UVC-LED equipment respectively. The curative effect of UVC-LED was determined by wound healing rate and histopathological changes. The expression of transforming growth factor-alpha (TGF-α), epidermal growth factor receptor (EGFR), cluster of differentiation 31 (CD31), cluster of differentiation 68 (CD68), vascular endothelial growth factor (VEGF) and the mRNA content of TGF-α, EGFR, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and VEGF were detected to clarify the mechanism of UVC-LED promoting wound healing.</p><p><strong>Results: </strong>Histopathology showed that both low-pressure mercury lamp and UVC-LED irradiation could promote skin wound healing, reduce the degree of inflammatory response, and promote angiogenesis. UVC-LED could better promote collagen fiber proliferation. UVC-LED promotes the expression of key cytokines in wound re-epithelialization by increasing the levels of TGF-α and EGFR, dynamically regulates wound healing by bidirectionally regulating the expression of inflammatory factors TNF-α and IL-6, promotes wound angiogenesis by increasing the levels of CD31 and VEGF, and reduces and regulates inflammation by increasing the number of macrophages by increasing CD68.</p><p><strong>Conclusions: </strong>254 nm UVC-LED can significantly promote skin wound healing in rats and play a role by regulating tissue regeneration cytokines, inflammatory factors and angiogenesis mechanisms.</p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lasers in Surgery and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/lsm.70030","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Significance: 254 nm short wave ultraviolet (UVC) emitted by low-pressure mercury lamp is effective in the treatment of skin wounds. With the emergence of deep ultraviolet light-emitting diodes (LED), it is expected to significantly expand the clinical indications of UVC. However, whether 254 nm UVC generated by LED light source can promote skin wound healing has not been reported.
Aim: To clarify the effect of 254 nm UVC-LED light source on promoting skin wound healing and explore the mechanism of UVC-LED promoting wound healing.
Approach: The full-thickness wound model of rat back skin was constructed, and the skin wounds of rats were treated with low-pressure mercury lamp and 254 nm UVC-LED equipment respectively. The curative effect of UVC-LED was determined by wound healing rate and histopathological changes. The expression of transforming growth factor-alpha (TGF-α), epidermal growth factor receptor (EGFR), cluster of differentiation 31 (CD31), cluster of differentiation 68 (CD68), vascular endothelial growth factor (VEGF) and the mRNA content of TGF-α, EGFR, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and VEGF were detected to clarify the mechanism of UVC-LED promoting wound healing.
Results: Histopathology showed that both low-pressure mercury lamp and UVC-LED irradiation could promote skin wound healing, reduce the degree of inflammatory response, and promote angiogenesis. UVC-LED could better promote collagen fiber proliferation. UVC-LED promotes the expression of key cytokines in wound re-epithelialization by increasing the levels of TGF-α and EGFR, dynamically regulates wound healing by bidirectionally regulating the expression of inflammatory factors TNF-α and IL-6, promotes wound angiogenesis by increasing the levels of CD31 and VEGF, and reduces and regulates inflammation by increasing the number of macrophages by increasing CD68.
Conclusions: 254 nm UVC-LED can significantly promote skin wound healing in rats and play a role by regulating tissue regeneration cytokines, inflammatory factors and angiogenesis mechanisms.
期刊介绍：
Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.
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