Single nucleotide polymorphisms in IFN-gamma, TNF-alpha, IL-6, IL-10, and TGF-beta in pulmonary and extrapulmonary tuberculosis in the State of Ceará, northeastern Brazil.

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2025-05-16 eCollection Date: 2025-01-01 DOI:10.1590/0074-02760240147

Roberta Dos Santos Silva Luiz, Thales Alves Campelo, Caroliny Soares Silva, Lucas de Lima Nogueira, Soraya de Oliveira Sancho, Ana Karolliny Alves da Silva, Cristiane Cunha Frota, Filipe Anibal Carvalho-Costa

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引用次数: 0

Abstract

Background: Single nucleotide polymorphisms (SNP) in genes encoding cytokines influence tuberculosis (TB) outcomes.

Objectives: To characterise genotypes of the SNPs IFN-gamma +874 T > A, TNF-alpha -308 G > A, IL-6 -174 G > C, IL-10 -1082A > G, TGF-beta codon 10 T > C, and TGF-beta codon 25 G > C in patients with pulmonary (PTB) and extrapulmonary TB (EPTB).

Methods: 82 PTB and 45 EPTB cases were compared, concerning genotype distribution of the mentioned SNPs, characterised via sequence-specific primer polymerase chain reaction (PCR).

Findings: Regarding IFN-gamma +874 T > A, AA genotype was the most frequent in both groups, TA was more frequent in PTB and TT in EPTB, with no statistical significance. For SNP TNF-alpha -308 G > A, GG was more frequent in both groups of patients. Regarding the IL-6 -174 G > C polymorphism, GG predominated in both groups, while CG and GG were significantly more frequent in patients with PTB and EPTB, respectively. Concerning IL-10 -1082 A > G, AA predominated in both PTB and EPTB. Concerning TGF-beta codon 10 T > C, CC predominated in PTB while TC predominated in EPTB, but the differences were not statistically significant. Genotype GG of TGF-beta codon 25 G > C predominated among PTB and EPTB patients.

Main conclusions: Except for IL-6, the genotype profile could not differentiate PTB and EPTB. Hence, the studied SNPs are not significantly associated with the extrapulmonary involvement of TB.

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巴西东北部塞埃尔州肺结核和肺外结核中ifn - γ、tnf - α、IL-6、IL-10和tgf - β的单核苷酸多态性

背景：细胞因子编码基因的单核苷酸多态性（SNP）影响结核病（TB）的预后。目的：探讨肺（PTB）和肺外结核（EPTB）患者中snp ifn - γ +874 T > A、tnf - α -308 G > A、IL-6 -174 G > C、IL-10 -1082A > G、tgf - β密码子10t > C和tgf - β密码子25g > C的基因型。方法：采用序列特异性引物聚合酶链反应（PCR）对82例肺结核和45例EPTB的snp基因型分布进行比较。结果：在ifn - γ +874 T > A中，AA基因型在两组中最常见，TA基因型在PTB中更常见，TT基因型在EPTB中更常见，差异无统计学意义。对于SNP tnf - α -308 G > A，两组患者中GG发生率更高。IL-6 -174 G > C多态性在两组中均以GG为主，而CG和GG在PTB和EPTB患者中分别更为常见。在IL-10 -1082 A > G中，AA在PTB和EPTB中均占主导地位。tgf - β密码子10t> C在PTB中以CC为主，在EPTB中以TC为主，但差异无统计学意义。tgf - β密码子25g > C基因型GG在肺结核和EPTB患者中占主导地位。主要结论：除IL-6外，基因型谱不能区分PTB和EPTB。因此，所研究的snp与结核肺外累及无显著相关性。

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.