Anionic Surfactant-Triggered Excimer Emission from Dipyrene-Based Amphiphilic Dyes for Wash-Free Cell Membrane Imaging.

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2025-05-20 DOI:10.1007/s10895-025-04304-z

Yongling Zheng, Bailin Guo, Enju Wang, Bingxin Lei

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引用次数: 0

Abstract

Compared to monomer emission, excimer emission usually exhibits red-shifted, broadened, and large Stokes-shifted characteristics, which offer distinct advantages in biological imaging. In this work, we developed a rational design strategy for excimer-based cell membrane fluorescent probes, and on this basis, three fluorescent dyes (BPVP-C3, BPVP-C5 and BPVP-C8) exhibiting tunable excimer/monomer emission were obtained by covalent coupling of two pyrene units. In sodium dodecyl sulfate micelles, these fluorophores show bright excimer emission, whereas in dilute aqueous solutions, they emit monomer-dominated fluorescence. We proposed that the anionic micellar environment promotes intramolecular excimer formation between coupled pyrene units due to its balanced amphiphilicity, while these dye molecules can freely swing in dilute aqueous solution. Among them, BPVP-C8 exhibited optimal excimer-monomer switching property. Leveraging this environment-responsive dual-emission, it was explored as a wash-free cell membrane probe for simultaneous dual-channel (green and red) imaging.

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阴离子表面活性剂引发的二芘基两亲性染料的准分子发射用于无水洗细胞膜成像。

与单体发射相比，准分子发射通常表现出红移、加宽和大斯托克斯位移特征，在生物成像中具有明显的优势。在这项工作中，我们开发了基于准分子的细胞膜荧光探针的合理设计策略，并在此基础上，通过两个芘单元的共价偶联得到了三种具有可调准分子/单体发射的荧光染料（BPVP-C3， BPVP-C5和BPVP-C8）。在十二烷基硫酸钠胶束中，这些荧光团显示出明亮的准分子发射，而在稀水溶液中，它们发出以单体为主的荧光。我们提出阴离子胶束环境由于其平衡的两亲性促进了偶联芘单元之间分子内准分子的形成，而这些染料分子可以在稀水溶液中自由摆动。其中，BPVP-C8具有最佳的准分子-单体开关性能。利用这种对环境敏感的双发射，研究人员将其作为一种免水洗的细胞膜探针，用于同时进行双通道（绿色和红色）成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.