Efficient biobutanol production via co-cultivation of Clostridium acetobutylicum and Bacillus cereus utilizing DES pretreated rice husk

IF 3.5 4区工程技术 Q3 ENERGY & FUELS

Biomass Conversion and Biorefinery Pub Date : 2024-10-16 DOI:10.1007/s13399-024-06225-6

A. Anuradha, Sudeepan Jayabalan, Swaraj Sengupta, Si-Yu Li, Muthu Kumar Sampath

{"title":"Efficient biobutanol production via co-cultivation of Clostridium acetobutylicum and Bacillus cereus utilizing DES pretreated rice husk","authors":"A. Anuradha, Sudeepan Jayabalan, Swaraj Sengupta, Si-Yu Li, Muthu Kumar Sampath","doi":"10.1007/s13399-024-06225-6","DOIUrl":null,"url":null,"abstract":"<div><p>Biobutanol, a promising biofuel with superior properties compared to ethanol, has garnered significant attention as an alternative to traditional fossil fuels. Recently, lignocellulosic biomasses (LCB) represent promising sustainable feedstock options for biorefineries aiming to produce renewable biofuels and biochemicals. Deep eutectic solvents (DES) are now recognized as an effective pretreatment method for lignocellulosic biomass, as they improve cellulose accessibility for subsequent hydrolysis and enhance fermentable sugar yield. DES has several benefits over conventional solvents, such as low toxicity and biodegradability which makes them appropriate for usage with a variety of lignocellulosic biomass. Therefore, in the present study, pretreatment of rice husk with deep eutectic solvent using choline chloride and urea (ChCl/U) was investigated for the production of biobutanol. This study also investigates the synergistic potential of coculturing <i>Bacillus cereus</i> and <i>Clostridium acetobutylicum</i> for enhanced biobutanol production. <i>B. cereus</i>, known for its robust metabolism and extracellular enzyme secretion, is paired with <i>C. acetobutylicum</i>, a proficient butanol producer. The coculture produced 4.7 ± 0.7 g/L of biobutanol. The coculture strategy aims to capitalize on the complementary metabolic capabilities of the two strains, facilitating improved substrate utilization and butanol production.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 10","pages":"15113 - 15121"},"PeriodicalIF":3.5000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomass Conversion and Biorefinery","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13399-024-06225-6","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENERGY & FUELS","Score":null,"Total":0}

引用次数: 0

Abstract

Biobutanol, a promising biofuel with superior properties compared to ethanol, has garnered significant attention as an alternative to traditional fossil fuels. Recently, lignocellulosic biomasses (LCB) represent promising sustainable feedstock options for biorefineries aiming to produce renewable biofuels and biochemicals. Deep eutectic solvents (DES) are now recognized as an effective pretreatment method for lignocellulosic biomass, as they improve cellulose accessibility for subsequent hydrolysis and enhance fermentable sugar yield. DES has several benefits over conventional solvents, such as low toxicity and biodegradability which makes them appropriate for usage with a variety of lignocellulosic biomass. Therefore, in the present study, pretreatment of rice husk with deep eutectic solvent using choline chloride and urea (ChCl/U) was investigated for the production of biobutanol. This study also investigates the synergistic potential of coculturing Bacillus cereus and Clostridium acetobutylicum for enhanced biobutanol production. B. cereus, known for its robust metabolism and extracellular enzyme secretion, is paired with C. acetobutylicum, a proficient butanol producer. The coculture produced 4.7 ± 0.7 g/L of biobutanol. The coculture strategy aims to capitalize on the complementary metabolic capabilities of the two strains, facilitating improved substrate utilization and butanol production.

Graphical abstract

查看原文本刊更多论文

利用DES预处理稻壳共同培养乙酰丁酸梭菌和蜡样芽孢杆菌高效生产生物丁醇

生物丁醇作为一种具有比乙醇更优越性能的生物燃料，作为传统化石燃料的替代品而备受关注。最近，木质纤维素生物质（LCB）为旨在生产可再生生物燃料和生物化学品的生物精炼厂提供了有前途的可持续原料选择。深共晶溶剂（DES）现在被认为是木质纤维素生物质的一种有效的预处理方法，因为它们提高了纤维素对后续水解的可及性，并提高了可发酵糖的产量。与传统溶剂相比，DES有几个优点，例如低毒和可生物降解性，这使得它们适合与各种木质纤维素生物质一起使用。因此，本研究采用氯化胆碱和尿素（ChCl/U）作为深度共熔溶剂，对稻壳进行预处理，制备生物丁醇。本研究还探讨了蜡样芽孢杆菌和乙酰丁酸梭菌共培养提高生物丁醇产量的协同潜力。蜡样芽孢杆菌以其强大的代谢和细胞外酶分泌而闻名，与C. acetobutylicum配对，C. acetobutylicum是一种熟练的丁醇生产商。共培养产生4.7±0.7 g/L的生物丁醇。共培养策略旨在利用两种菌株的互补代谢能力，促进提高底物利用率和丁醇产量。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomass Conversion and Biorefinery Energy-Renewable Energy, Sustainability and the Environment

CiteScore

7.00

自引率

15.00%

发文量

1358

期刊介绍： Biomass Conversion and Biorefinery presents articles and information on research, development and applications in thermo-chemical conversion; physico-chemical conversion and bio-chemical conversion, including all necessary steps for the provision and preparation of the biomass as well as all possible downstream processing steps for the environmentally sound and economically viable provision of energy and chemical products.