Optimization of bioethanol production from reducing sugar in stress tolerance by GSH: GSSG cycle in S. cerevisiae

IF 3.5 4区工程技术 Q3 ENERGY & FUELS

Biomass Conversion and Biorefinery Pub Date : 2024-10-16 DOI:10.1007/s13399-024-06247-0

Huma Gulzar, Tuba Tariq, Iqra Kainat, Huiqiang Lou, Mansour Ghorbanpour, Ghazala Mustafa, Murtaza Hasan

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引用次数: 0

Abstract

Bio-ethanol is the largest and most dominant biotechnology bio-fuel and Saccharomyces cerivicae is the most favored microorganism employed for its industrial production. However, obtaining maximum yields from an ethanol fermentation and reducing ethanol fermentation stress remain a technical challenge. Usage of food biomass for producing bio-fuel caused food shortage, and conversion of waste lignocellulosic biomass into bio-fuel was costly and affecting the economy of a country. Here, we use fungal biomass for production of reducing sugars and fermentation of reducing sugar into bioethanol. To get better results, optimization of reactant such as fungal biomass by chemical and enzymatic pretreatment. The highest reducing sugars obtained was 1.98 g/100 mL with 99% sugar yield by sample treated with α-amylase (50FBGU). All samples were fermented using S. cerivicae (yeast) and highest bioethanol produced was 6.07% by a sample treated with α-amylase (50FBGU). This study revealed that fungal biomass pretreated enzymatically and chemically give higher production of reducing sugars and bioethanol and explored the sub-cellular distribution and response of intracellular glutathione with ethanol fermentation stresses include ROS potentially impact on the efficiency of yeast cell growth and metabolism; conferring stress tolerance by GSH:GSSG cycle to increase catalase activity being deployed at industrial scale for the optimization and potential to improve fermentation efficiency.

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酿酒酵母GSH - GSSG循环中抗逆性还原糖生产生物乙醇的优化

生物乙醇是生物技术中规模最大、最具优势的生物燃料，酿酒酵母是工业生产中最受青睐的微生物。然而，从乙醇发酵中获得最大产量和降低乙醇发酵应力仍然是一个技术挑战。利用粮食生物质生产生物燃料造成粮食短缺，并且将废弃的木质纤维素生物质转化为生物燃料成本高昂，影响一个国家的经济。在这里，我们利用真菌生物量生产还原糖和发酵还原糖成生物乙醇。为获得较好的效果，对真菌生物量等反应物进行了化学预处理和酶法预处理。α-淀粉酶（50FBGU）处理得到的还原糖最高为1.98 g/100 mL，产糖率为99%。所有样品均采用酵母发酵，α-淀粉酶（50FBGU）处理的样品生物乙醇产量最高，为6.07%。本研究揭示了酵母菌生物量经酶和化学预处理后可提高还原糖和生物乙醇的产量，并探讨了细胞内谷胱甘肽的亚细胞分布和对乙醇发酵应激的响应，包括ROS对酵母细胞生长和代谢效率的潜在影响；通过GSH:GSSG循环提高过氧化氢酶活性，并在工业规模上进行优化，提高发酵效率。

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来源期刊

Biomass Conversion and Biorefinery Energy-Renewable Energy, Sustainability and the Environment

CiteScore

7.00

自引率

15.00%

发文量

1358

期刊介绍： Biomass Conversion and Biorefinery presents articles and information on research, development and applications in thermo-chemical conversion; physico-chemical conversion and bio-chemical conversion, including all necessary steps for the provision and preparation of the biomass as well as all possible downstream processing steps for the environmentally sound and economically viable provision of energy and chemical products.