Zuoping Li, Jiaru Zhang, Shiwan You, Jing Zhang, Zhenzhen Xie, Na Zhao, Shiguo Sun
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引用次数: 0
Abstract
Neuropilin-1 (NRP-1) is overly expressed on the membrane of cervical cancer cells, which is indispensable for cell internalization. NRP-1 achieves the goal of targeting cervical cancer cells with tumor homing peptide (THPs) in C-terminal (CendR) pathway. By this feature, the ligand LyP-2 peptide of NRP-1 with high affinity (also containing CendR motif) was functionalized on the surface of liposome (Lipo@LyP-2) to mediate its tumor homing. Under TEM, Lipo@LyP-2 is a smooth global body, whose diameter was less than 200 nm. Lipo@LyP-2 showed enhanced cell uptake in cervical cancer cells associated with human papillomavirus type 16 (HPV16) by the Confocal laser scanning microscope (CLSM). In the MTT assay, Lipo@LyP-2/SAHA/CDDP loaded with cisplatin (CDDP) and vorinostat (SAHA) also showed significant toxicity via increasing pro-apoptotic factors and decreasing anti-apoptotic factors. In addition, Western blot assay found that SAHA enhanced the intra-tumoral level of MHC class I chain-associated protein A (MICA) in cervical cancer, thus enhancing the sensitivity of cervical cancer cells to cytolysis mediated by natural killer (NK) cells. These results strongly indicate that LyP-2 functionalized Lipo/SAHA/CDDP can improve the targeting of CDDP and SAHA to SiHa cells and improve the therapeutic efficiency.
期刊介绍:
The journal publishes the following types of papers: (a) original and important research;
(b) authoritative comprehensive reviews or short overviews of topics of current
interest; (c) brief but urgent communications on new significant research; and (d)
commentaries intended to foster the exchange of innovative or provocative ideas, and
to encourage dialogue, amongst researchers working in different cluster
disciplines.