The transcription factor IID subunit Taf13 is dispensable for TATA binding protein promoter recruitment and RNA polymerase II transcription

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-05-05 DOI:10.1016/j.isci.2025.112286

Igor Martianov , Stephanie Le Gras , Guillaume Davidson , Irwin Davidson

引用次数: 0

Abstract

The multiprotein complex TFIID, comprising the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs), is an essential component of the RNA polymerase II (Pol II) preinitiation complex (PIC). Cryo-electron microscopy studies suggested a critical role of the TAF11-TAF13 heterodimer in TBP promoter deposition upstream of the transcription start site. To investigate this hypothesis, we inactivated the gene encoding Taf13 in mice and embryonic stem cells (ESCs). Taf13-null embryos implant and survive until E6.5, but fail to undergo gastrulation, while Taf13-null ESCs are viable, but fail to form embryoid bodies and differentiate. Taf13 loss had little effect on TFIID integrity and led to only a mild reduction of TBP promoter recruitment, but led to altered PIC formation and globally reduced Pol II recruitment. Thus, the Taf11-Taf13 heterodimer is not essential for TBP/TFIID recruitment, revealing plasticity in the pathways of PIC formation.

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转录因子IID亚基Taf13对于TATA结合蛋白启动子募集和RNA聚合酶II转录是必不可少的

多蛋白复合物TFIID由TATA结合蛋白（TBP）和13个TBP相关因子（TAFs）组成，是RNA聚合酶II （Pol II）预起始复合物（PIC）的重要组成部分。低温电镜研究表明，TAF11-TAF13异源二聚体在转录起始位点上游的TBP启动子沉积中起着关键作用。为了验证这一假说，我们在小鼠和胚胎干细胞（ESCs）中灭活了编码Taf13的基因。Taf13-null胚胎植入并存活至E6.5，但不能发生原肠胚形成，而Taf13-null胚胎可存活，但不能形成胚状体并分化。Taf13缺失对TFIID完整性的影响很小，仅导致TBP启动子招募轻微减少，但导致PIC形成改变和全局Pol II招募减少。因此，Taf11-Taf13异源二聚体对于TBP/TFIID的募集并不是必需的，这揭示了PIC形成途径的可塑性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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