Functional divergence of plant SCAR/WAVE proteins is determined by intrinsically disordered regions

IF 11.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2025-05-21 DOI:10.1126/sciadv.adt6107

Sabine Brumm, Aleksandr Gavrin, Matthew Macleod, Guillaume Chesneau, Annika Usländer, Sebastian Schornack

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Abstract

Dynamic actin cytoskeleton reorganization enables plant developmental processes requiring polarized transport such as root hair and leaf trichome formation. The SCAR/WAVE complex plays a crucial role in regulating these dynamics through ARP2/3-mediated actin branching. SCAR/WAVE genes occur as small families across a wide range of plant species, but whether and how they fulfill different functions remains unclear. We use a systematic chimera approach to define the differential functionality of two closely related Medicago truncatula SCAR proteins in plant development. We show that SCAR/WAVE contribution to M. truncatula root hair or Arabidopsis thaliana trichome formation is dependent on two central intrinsically disordered regions (IDRs). Differential functionalities of M. truncatula SCAR proteins were furthermore associated with the presence/absence of a 42–amino acid sequence within the IDR that affected protein stability. Through uncovering a molecular basis for functional differences, we advance our understanding of plant SCAR/WAVE complexes.

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植物SCAR/WAVE蛋白的功能分化是由内在无序区决定的

动态肌动蛋白细胞骨架重组使植物发育过程需要极化运输，如根毛和叶毛状体的形成。SCAR/WAVE复合体通过arp2 /3介导的肌动蛋白分支在调节这些动力学中起着至关重要的作用。SCAR/WAVE基因以小科的形式存在于广泛的植物物种中，但它们是否以及如何实现不同的功能尚不清楚。我们使用系统的嵌合体方法来定义两个密切相关的紫花苜蓿SCAR蛋白在植物发育中的差异功能。我们发现SCAR/WAVE对M. truncatula根毛或拟南芥毛形成的贡献依赖于两个中心的内在无序区（IDRs）。此外，短尾霉SCAR蛋白的不同功能与IDR中42个氨基酸序列的存在/缺失有关，这影响了蛋白质的稳定性。通过揭示功能差异的分子基础，我们进一步了解了植物SCAR/WAVE复合物。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.