Human IgG responses to Anopheles gambiae immunogenic salivary proteins in urban and rural populations of Burkina Faso: biomarkers of exposure to malaria vector bites.
Michaël Kaboré, Yéri Esther Hien, Dado Jean Noël Koussé, Fatimata Thiombiano, Mireille Ouédraogo, Abdoul Rahamani Nikiema, Enock Ibrango, Nicolas Ouédraogo, Mariama K Cherif, Sylvain Ilboudo, Toussaint Rouamba, Guillaume Sylvestre Sanou
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Abstract
Background: Malaria control would be greatly facilitated by the development of new tools for rapidly assessing malaria transmission intensity. In malaria-endemic areas such as Burkina Faso, human populations are frequently exposed to immunomodulatory salivary components injected during mosquito blood feeding. Numerous studies have examined parasite immunity; however, there are few data available on vector immunity as a means of assessing malaria transmission in sub-Saharan Africa. The present study aims to compare IgG-specific response to salivary gland extracts (SGE) of Anopheles gambiae (An. gambiae) in populations living in urban and rural areas in Burkina Faso.
Methods: A cross-sectional descriptive study was carried out in two sites, Ouagadougou city and Sapouy village, where blood samples (n = 676) from children (0-15 years) and adults were collected. After An. gambiae salivary protein isolation, the antibody (IgG) response to those SGE was evaluated by enzyme-linked immunosorbent assay (ELISA), representing a proxy of Anopheles exposure. The difference in antibody concentrations between groups was tested using parametric tests (Student's t-test and analysis of variance [ANOVA]) and the nonparametric Mann-Whitney U (Wilcoxon rank-sum) test. All differences were considered significant at P < 0.05.
Results: The study population consisted of 63.0% males and 37.0% females (average age = 31.2 ± 17.8 years). IgG antibodies against An. gambiae salivary protein were detected in all study participants. Urban participants demonstrated a greater mean IgG level to An. gambiae bites than rural (P < 0.0001). The mean IgG level was higher in secondary school children compared with primary school children (P < 0.0001). Organic cotton farmers held higher IgG to An. gambiae bites than conventional cotton farmers (P = 0.0027).
Conclusions: The evaluation of IgG specific to mosquito salivary gland extracts as immunological biomarkers in populations in Burkina Faso allowed us to show that the human anti-SGE IgG level to An. gambiae bites is strongly influenced by the living environment and the use of insecticides in agriculture.
期刊介绍：
Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish.
Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
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