MZB1 regulates the immune microenvironment and inhibits ovarian cancer cell migration.

IF 1.6 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Open Medicine Pub Date : 2025-05-13 eCollection Date: 2025-01-01 DOI:10.1515/med-2025-1174

Mingyue Zhu, Guxin Zhou, Fangyuan Chang, Jin Liu

{"title":"MZB1 regulates the immune microenvironment and inhibits ovarian cancer cell migration.","authors":"Mingyue Zhu, Guxin Zhou, Fangyuan Chang, Jin Liu","doi":"10.1515/med-2025-1174","DOIUrl":null,"url":null,"abstract":"Background: The immune microenvironment of ovarian cancer is crucial in its progression. Recent studies highlight the significant role of MZB1 in shaping the tumor immune microenvironment (TIME), although its specific function in ovarian cancer remains unclear.Materials and methods: We analyzed 381 ovarian cancer samples from the TCGA database, along with additional samples from GEO and single-cell datasets. Differentially expressed genes (DEGs) were identified using DESeq2. Various algorithms were applied to assess the relationship between MZB1 and the TIME. Cell proliferation was measured using the CCK-8 assay, while cell migration was evaluated using the wound healing assay. Furthermore, a nomogram predicting overall survival was developed based on multivariable Cox regression results.Results: Our results indicated a positive correlation between high MZB1 expression and improved clinical prognosis. Additionally, higher MZB1 expression was linked to increased immune cell infiltration within the TIME. Elevated MZB1 levels inhibited the migration and proliferation of SKOV3 cells. The nomogram's C-index was 0.702, and its calibration curve demonstrated good calibration, indicating satisfactory discrimination and accuracy in predicting patient outcomes.Conclusions: This study comprehensively analyzes MZB1's role in the TIME of ovarian cancer. MZB1 is a promising prognostic marker and a potential target for personalized treatment.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251174"},"PeriodicalIF":1.6000,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12086629/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1515/med-2025-1174","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Background: The immune microenvironment of ovarian cancer is crucial in its progression. Recent studies highlight the significant role of MZB1 in shaping the tumor immune microenvironment (TIME), although its specific function in ovarian cancer remains unclear.

Materials and methods: We analyzed 381 ovarian cancer samples from the TCGA database, along with additional samples from GEO and single-cell datasets. Differentially expressed genes (DEGs) were identified using DESeq2. Various algorithms were applied to assess the relationship between MZB1 and the TIME. Cell proliferation was measured using the CCK-8 assay, while cell migration was evaluated using the wound healing assay. Furthermore, a nomogram predicting overall survival was developed based on multivariable Cox regression results.

Results: Our results indicated a positive correlation between high MZB1 expression and improved clinical prognosis. Additionally, higher MZB1 expression was linked to increased immune cell infiltration within the TIME. Elevated MZB1 levels inhibited the migration and proliferation of SKOV3 cells. The nomogram's C-index was 0.702, and its calibration curve demonstrated good calibration, indicating satisfactory discrimination and accuracy in predicting patient outcomes.

Conclusions: This study comprehensively analyzes MZB1's role in the TIME of ovarian cancer. MZB1 is a promising prognostic marker and a potential target for personalized treatment.

Abstract Image

查看原文本刊更多论文

MZB1调节免疫微环境，抑制卵巢癌细胞迁移。

背景：卵巢癌的免疫微环境在其发展过程中起着至关重要的作用。最近的研究强调了MZB1在形成肿瘤免疫微环境（TIME）中的重要作用，尽管其在卵巢癌中的具体功能尚不清楚。材料和方法：我们分析了来自TCGA数据库的381例卵巢癌样本，以及来自GEO和单细胞数据集的额外样本。差异表达基因（DEGs）用DESeq2进行鉴定。采用各种算法评估MZB1与TIME之间的关系。使用CCK-8法测量细胞增殖，使用伤口愈合法评估细胞迁移。此外，根据多变量Cox回归结果，建立了预测总生存期的nomogram。结果：我们的研究结果表明MZB1高表达与改善临床预后呈正相关。此外，MZB1的高表达与TIME内免疫细胞浸润增加有关。MZB1水平升高可抑制SKOV3细胞的迁移和增殖。nomogram C-index为0.702，其校正曲线具有良好的校正性，在预测患者预后方面具有良好的判别性和准确性。结论：本研究全面分析了MZB1在卵巢癌TIME中的作用。MZB1是一种很有前景的预后标记物，也是个性化治疗的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Open Medicine Medicine-General Medicine

CiteScore

3.00

自引率

0.00%

发文量

153

审稿时长

20 weeks

期刊介绍： Open Medicine is an open access journal that provides users with free, instant, and continued access to all content worldwide. The primary goal of the journal has always been a focus on maintaining the high quality of its published content. Its mission is to facilitate the exchange of ideas between medical science researchers from different countries. Papers connected to all fields of medicine and public health are welcomed. Open Medicine accepts submissions of research articles, reviews, case reports, letters to editor and book reviews.