Electroacupuncture Improves Intestinal Ischemia/Reperfusion Injury via cGAS-STING Pathway.

IF 2.9 3区医学 Q1 CLINICAL NEUROLOGY

Neurogastroenterology and Motility Pub Date : 2025-10-01 Epub Date: 2025-05-20 DOI:10.1111/nmo.70066

Yali Zhu, Chao Fang, Shuxian Chen, Ling Xiao, Jingcheng Wang, Yun Wu, Jia Zhan

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引用次数: 0

Abstract

Background: Electroacupuncture (EA) is a treatment method that stimulates acupuncture points to mobilize resistance to disease and bioelectricity to achieve therapeutic effects. Related studies have found that EA has a certain therapeutic effect on ischemia/reperfusion (I/R) injury of some organs. Among the internal organs, the intestine is the most sensitive to ischemic injury. Therefore, this study aims to preliminarily explore the effects and mechanisms of EA on intestinal I/R injury through animal experiments and provide a theoretical basis for the clinical treatment of intestinal I/R injury.

Methods: In this study, the intestinal I/R injury model was established by occluding the superior mesenteric artery (SMA) of mice for 45 min and then reperfusing for 2 h. By applying EA to the bilateral Zusanli in mice, this study aimed to investigate its therapeutic effects on intestinal I/R injury.

Key results: The results showed that EA can improve the survival and nutritional status of mice with intestinal I/R injury. EA alleviated intestinal tissue damage and the inflammatory response induced by intestinal I/R injury and protected the integrity of intestinal epithelial cells and intestinal transport function. Inflammatory factors induced by intestinal I/R injury were associated with increased levels of STING, and EA could inhibit the increase in STING protein levels and the phosphorylation of downstream factors IRF3 and TBK1 of the cGAS-STING pathway.

Conclusions: EA can alleviate intestinal tissue damage caused by intestinal I/R in mice, protect the intestinal epithelial barrier, and improve intestinal transit function. The underlying mechanism may involve inhibition of cGAS-STING pathway activation and reduction of the inflammatory response.

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电针通过cGAS-STING通路改善肠缺血再灌注损伤。

背景：电针是一种通过刺激穴位调动对疾病的抵抗力和生物电来达到治疗效果的治疗方法。相关研究发现，EA对部分器官的缺血再灌注（I/R）损伤有一定的治疗作用。在内脏器官中，肠道对缺血性损伤最为敏感。因此，本研究旨在通过动物实验初步探讨EA对肠道I/R损伤的作用及机制，为临床治疗肠道I/R损伤提供理论依据。方法：采用阻断小鼠肠系膜上动脉（SMA） 45 min，再灌注2 h的方法建立肠I/R损伤模型。本研究通过对小鼠双侧足三里进行EA处理，探讨其对肠道I/R损伤的治疗作用。关键结果：结果表明，EA可改善肠I/R损伤小鼠的生存和营养状况。EA可减轻肠I/R损伤引起的肠组织损伤和炎症反应，保护肠上皮细胞的完整性和肠运输功能。肠I/R损伤诱导的炎症因子与STING水平升高相关，EA可抑制STING蛋白水平升高以及cGAS-STING通路下游因子IRF3和TBK1的磷酸化。结论：EA可减轻肠道I/R对小鼠肠道组织的损伤，保护肠道上皮屏障，改善肠道转运功能。其潜在机制可能涉及抑制cGAS-STING通路激活和减少炎症反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neurogastroenterology and Motility 医学-临床神经学

CiteScore

7.80

自引率

8.60%

发文量

178

审稿时长

3-6 weeks

期刊介绍： Neurogastroenterology & Motility (NMO) is the official Journal of the European Society of Neurogastroenterology & Motility (ESNM) and the American Neurogastroenterology and Motility Society (ANMS). It is edited by James Galligan, Albert Bredenoord, and Stephen Vanner. The editorial and peer review process is independent of the societies affiliated to the journal and publisher: Neither the ANMS, the ESNM or the Publisher have editorial decision-making power. Whenever these are relevant to the content being considered or published, the editors, journal management committee and editorial board declare their interests and affiliations.