Full-spectrum Cannabis sativa extract enhances gut-peripheral organ integrity after experimental ischemic stroke.

IF 4.6 2区医学 Q2 IMMUNOLOGY

Inflammopharmacology Pub Date : 2025-05-19 DOI:10.1007/s10787-025-01775-1

Solange de Souza Stork, Khiany Mathias, Fernanda Gava, Larissa Joaquim, David Dos Santos, Anita Dal Bó Tiscoski, Sandra Bonfante, Yasmin Ribeiro Strickert, Richard Simon Machado, Helena Mafra Martins, Jéssica Schaefer Chaves, Jaqueline Generoso, Lucineia Gainski Danielski, Amanda Della Giustina, Rahisa Scussel, Rafael Bitencourt, Josiel Mileno Mack, Mariana Pereira de Souza Goldim, Ricardo Andrez Machado-de-Ávila, Tatiana Barichello, Franciane Bobinski, Fabricia Petronilho

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引用次数: 0

Abstract

Objective: This study aims to investigate the impact of full-spectrum Cannabis sativa extract (FSC) treatment on gut and peripheral organ protection after ischemic stroke.

Main methods: Male Wistar rats were subjected to 60-min middle cerebral artery occlusion (MCAO) or sham surgery, and received FSC (15 or 30 mg/kg) or coconut oil by gavage at different time points post-MCAO. After 72 h, neurological score, infarct volume, blood cell count, thymus, spleen and adrenal gland size and weight, serum corticosterone, intestinal permeability, oxidative stress, and inflammatory cytokines in peripheral organs were assessed.

Key findings: The results show a significant improvement in neurological deficits, suggesting the therapeutic potential of FSC in post-stroke recovery. Additionally, a reduction in body mass, a decrease in blood cells related to the immune response, and atrophy of lymphoid organs, lower corticosterone levels, and reduced intestinal permeability were observed. FSC treatment also demonstrated a crucial role in protecting against oxidative stress and post-stroke lung inflammation.

Significance: The discovery of the positive impacts of FSC in this study represents an entry point for new explorations and perspectives within this field. With latent potential, these findings have the power to shape clinical research, especially in the realm of neurodegenerative diseases and innovative therapies. Therefore, the results highlight the promising role of FSC, paving the way for more effective and transformative clinical interventions.

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全谱大麻提取物增强实验性缺血性脑卒中后肠道外周器官的完整性。

目的：探讨全谱大麻提取物（FSC）对缺血性脑卒中后肠道和外周器官保护的影响。主要方法：雄性Wistar大鼠进行大脑中动脉闭塞（MCAO）或假手术60 min， MCAO后不同时间点灌胃FSC（15、30 mg/kg）或椰子油。72h后，评估神经学评分、梗死体积、血细胞计数、胸腺、脾脏和肾上腺的大小和重量、血清皮质酮、肠通透性、氧化应激和外周器官炎症因子。主要发现：结果显示神经功能缺损显著改善，提示FSC在卒中后恢复中的治疗潜力。此外，还观察到体重减少、与免疫反应相关的血细胞减少、淋巴器官萎缩、皮质酮水平降低和肠通透性降低。FSC治疗在防止氧化应激和中风后肺部炎症方面也发挥了至关重要的作用。意义：本研究中FSC的积极影响的发现为该领域的新探索和新视角提供了一个切入点。这些发现具有潜在的潜力，有能力塑造临床研究，特别是在神经退行性疾病和创新疗法领域。因此，结果突出了FSC的前景作用，为更有效和变革性的临床干预铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

8.00

自引率

3.40%

发文量

200

期刊介绍： Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]