Multi-omics analysis of the mechanisms of abundant theacrine and EGCG3"Me in tea (Camellia sinensis).

IF 4.3 2区生物学 Q1 PLANT SCIENCES

BMC Plant Biology Pub Date : 2025-05-19 DOI:10.1186/s12870-025-06691-8

Yanyu Zhu, Mengya Gu, Wentao Yu, Longhua Liao, Shuilian Gao, Shuyan Wang, Hongzheng Lin, Wenjing Gui, Youliang Zhou, Zhiming Chen, Jingde Zeng, Naixing Ye

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引用次数: 0

Abstract

Theacrine and epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me) are notable secondary metabolites in tea (Camellia sinensis), celebrated for their unique flavors and significant health effects. Theacrine has a mild effect on nerve stimulation, while EGCG3"Me exhibits better stability, higher oral bioavailability and stronger biological activity. However, tea plant varieties naturally rich in both theacrine and EGCG3"Me are rare. This study unveils a unique tea variety 'Anxi kucha', which is abundant in both theacrine and EGCG3"Me. Through integrated transcriptome-proteome-metabolome analysis, SAMS3, APRT1, IMPDH, and TCS1 were identified as critical enzymes for theacrine synthesis; while CHI1, CHI2, FLS2 and LAR1 were key for EGCG3"Me synthesis. Additionally, transcription factor analysis revealed that MYB4 and bHLH74 were positively correlated with the contents of theacrine and EGCG3"Me. This study provides valuable materials for further exploring theacrine and EGCG3"Me in tea plants, and establishes a theoretical basis for their biosynthesis.

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茶（Camellia sinensis）中分泌丰富茶碱和EGCG3"Me机制的多组学分析。

茶碱和表没食子儿茶素-3- o -（3- o -甲基）没食子酸酯（EGCG3"Me）是茶（茶树）中显著的次级代谢物，以其独特的风味和显著的健康作用而闻名。茶碱对神经的刺激作用较轻，而EGCG3“Me具有较好的稳定性、较高的口服生物利用度和较强的生物活性。然而，天然富含茶碱和EGCG3“Me的茶树品种是罕见的。本研究揭示了一种独特的茶品种“安溪库茶”，它富含acrine和EGCG3“Me”。通过转录组-蛋白质组-代谢组综合分析，SAMS3、APRT1、IMPDH和TCS1被鉴定为合成腺嘌呤的关键酶；而CHI1、CHI2、FLS2和LAR1是EGCG3“Me合成的关键。此外，转录因子分析显示MYB4和bHLH74与theacrine和EGCG3“Me”的含量呈正相关。本研究为进一步探索茶树中的茶碱和EGCG3“Me提供了有价值的材料，并为其生物合成奠定了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Plant Biology 生物-植物科学

CiteScore

8.40

自引率

3.80%

发文量

539

审稿时长

3.8 months

期刊介绍： BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.