Gold nanorods non-functionalised and associated with gallic acid exhibit activity against non-albicans Candida species.

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biofouling Pub Date : 2025-05-01 Epub Date: 2025-05-19 DOI:10.1080/08927014.2025.2504026
Paulo Henrique Fonseca Carmo, Maria Fernanda Siqueira Fernandes da Costa, Anna Carolina Pinheiro Lage, Maíra Terra Garcia, Juliana Campos Junqueira
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引用次数: 0

Abstract

Strategies focusing on natural compounds and nanotechnology have been explored to overcome the limitations of conventional therapies in managing Candida infections. In this context, metal nanoparticles, both non-functionalised and combined with gallic acid, may offer a promising alternative. This study investigated the effects of gold nanoparticles non-functionalised (AuNp) and associated with gallic acid (AuNpGA) against planktonic cells and biofilms of Nakaseomyces glabratus, Pichia kudriavzevii, Candida parapsilosis, and Candida tropicalis. Both AuNp and AuNpGA inhibited the growth of all strains at 1.56 µg/mL and exhibited fungicidal effects at concentrations ranging from 1.56 to 3.12 µg/mL. The time-kill curve revealed that AuNpGA and AuNp completely inhibited the viability of all strains in planktonic cultures at 8 and 24 h, respectively, exhibiting greater antifungal activity compared to fluconazole. Treatment with AuNp increased ROS production against N. glabratus and P. kudriavzevii. Oxidative stress was enhanced against all strains after treatment with AuNpGA, and exposure to this compound reduced ergosterol levels of P. kudriavzevii and C. parapsilosis. Furthermore, AuNpGA and AuNp significantly decreased the viability of all Candida biofilms at 7.8 and 15.6 µg/mL, respectively. In summary, both gold nanoparticles exhibited activity against planktonic cells and biofilms, suggesting their potential as agents for treating Candida infections.

与没食子酸相关的非功能化金纳米棒具有抗非白色念珠菌的活性。
人们已经探索了以天然化合物和纳米技术为重点的策略,以克服传统疗法在管理念珠菌感染方面的局限性。在这种情况下,金属纳米颗粒,非功能化和与没食子酸结合,可能提供一个有前途的替代品。本研究研究了非功能化金纳米颗粒(AuNp)和与没食子酸相关的金纳米颗粒(AuNpGA)对裸毛中aseomyces glabratus, Pichia kudriavzevii, parapsilosis假丝酵母和热带假丝酵母浮游细胞和生物膜的影响。在1.56µg/mL浓度范围内,AuNp和AuNpGA均能抑制所有菌株的生长;在1.56 ~ 3.12µg/mL浓度范围内,AuNp和AuNpGA均表现出杀真菌作用。时间杀伤曲线显示,AuNpGA和AuNp分别在8 h和24 h完全抑制所有菌株在浮游培养中的活力,比氟康唑表现出更强的抗真菌活性。ununp处理增加了对光秃稻和库氏稻的活性氧生成。经AuNpGA处理后,所有菌株的氧化应激均增强,暴露于该化合物可降低P. kudriavzevii和C. parapsilosis的麦角甾醇水平。此外,AuNpGA和AuNp分别以7.8µg/mL和15.6µg/mL显著降低了所有念珠菌生物膜的活力。总之,这两种金纳米颗粒都表现出对浮游细胞和生物膜的活性,表明它们有可能作为治疗念珠菌感染的药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biofouling
Biofouling 生物-海洋与淡水生物学
CiteScore
5.00
自引率
7.40%
发文量
57
审稿时长
1.7 months
期刊介绍: Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
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