Frequency and Distribution of KIR Genotypes of Donors-Recipient Pairs in the Haploidentical Haematopoietic Stem Cell Transplantation Setting: Collaborative Study by the Spanish Working Group in Histocompatibility and Transplant Immunology (GETHIT) and the Spanish Haematopoietic Transplantation and Cell Therapy Group (GETH-TC)

IF 5.9 4区医学 Q2 CELL BIOLOGY

HLA Pub Date : 2025-05-21 DOI:10.1111/tan.70248

Mar Luis-Hidalgo, Dolores Planelles, José Luis Piñana, Juan Carbonell, Paula Amat, Inés Gómez-Seguí, Manuel Guerreiro, Abelardo Caballero, Alberto Torío, Mª. Jesús Pascual-Cascón, Francisco Boix, Luis Marín Rubio, Lourdes Vázquez, Natalia Martínez-Pomar, Vanesa Cunill, Antonia Sampol, Julio Ochoa, Cristina Arbona, Carlos Solano

{"title":"Frequency and Distribution of KIR Genotypes of Donors-Recipient Pairs in the Haploidentical Haematopoietic Stem Cell Transplantation Setting: Collaborative Study by the Spanish Working Group in Histocompatibility and Transplant Immunology (GETHIT) and the Spanish Haematopoietic Transplantation and Cell Therapy Group (GETH-TC)","authors":"Mar Luis-Hidalgo, Dolores Planelles, José Luis Piñana, Juan Carbonell, Paula Amat, Inés Gómez-Seguí, Manuel Guerreiro, Abelardo Caballero, Alberto Torío, Mª. Jesús Pascual-Cascón, Francisco Boix, Luis Marín Rubio, Lourdes Vázquez, Natalia Martínez-Pomar, Vanesa Cunill, Antonia Sampol, Julio Ochoa, Cristina Arbona, Carlos Solano","doi":"10.1111/tan.70248","DOIUrl":null,"url":null,"abstract":"<div>\n \n There is limited information regarding the influence of KIR genotype, compared to the HLA system, in haploidentical haematopoietic stem cell transplantation (haplo-HSCT). This study aimed to determine the frequencies of KIR genotypes in Spanish haematologic patients undergoing haplo-HSCT. A study was conducted on 113 oncohaematological patients and their donors, treated across five centres that are members of the Spanish Working Group in Histocompatibility and Transplant Immunology (GETHIT) and the Spanish Haematopoietic Transplantation and Cell Therapy Group (GETH-TC). KIR typing was performed using PCR-rSSO or PCR-SSP. KIR genotypes were identified using the KIR Allele Frequency Net Database. Among donors, the most frequent KIR genotypes were Type 1 (28.3%), Type 2 (12.4%) and Type 4 (10.6%). In patients, Genotypes 1 (23.9%), 4 (23%) and 2 (14.2%) were most prevalent. Donors exhibited AA centromeric (46%) and telomeric (59.3%) types, while patients had a higher AB centromeric frequency (52.2%). Differences were observed in the BB centromeric type (3.5% patients; 16.8% donors, p = 0.002). The AB KIR genotype was the most common (70.8% donors; 75.2% patients). Most were classified as ‘neutral’ (61.9% donors; 73.5% patients). B-content score1 was the most common (48.7% patients; 33.6% donors). Notably, classification as best was rare (2.7% patients; 16.8% donors, p = 0.002). The study highlights the distribution of KIR genotypes in haplo-HSCT patients and donors, with Genotypes 1, 2 and 4 being the most prevalent. AB KIR genotypes and B-content score 1 were dominant. Moreover, KIR genotypes ID may serve as criteria for future investigation about the immunogenetic predisposition to malignant haematological diseases.\n </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"105 5","pages":""},"PeriodicalIF":5.9000,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"HLA","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tan.70248","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

There is limited information regarding the influence of KIR genotype, compared to the HLA system, in haploidentical haematopoietic stem cell transplantation (haplo-HSCT). This study aimed to determine the frequencies of KIR genotypes in Spanish haematologic patients undergoing haplo-HSCT. A study was conducted on 113 oncohaematological patients and their donors, treated across five centres that are members of the Spanish Working Group in Histocompatibility and Transplant Immunology (GETHIT) and the Spanish Haematopoietic Transplantation and Cell Therapy Group (GETH-TC). KIR typing was performed using PCR-rSSO or PCR-SSP. KIR genotypes were identified using the KIR Allele Frequency Net Database. Among donors, the most frequent KIR genotypes were Type 1 (28.3%), Type 2 (12.4%) and Type 4 (10.6%). In patients, Genotypes 1 (23.9%), 4 (23%) and 2 (14.2%) were most prevalent. Donors exhibited AA centromeric (46%) and telomeric (59.3%) types, while patients had a higher AB centromeric frequency (52.2%). Differences were observed in the BB centromeric type (3.5% patients; 16.8% donors, p = 0.002). The AB KIR genotype was the most common (70.8% donors; 75.2% patients). Most were classified as ‘neutral’ (61.9% donors; 73.5% patients). B-content score1 was the most common (48.7% patients; 33.6% donors). Notably, classification as best was rare (2.7% patients; 16.8% donors, p = 0.002). The study highlights the distribution of KIR genotypes in haplo-HSCT patients and donors, with Genotypes 1, 2 and 4 being the most prevalent. AB KIR genotypes and B-content score 1 were dominant. Moreover, KIR genotypes ID may serve as criteria for future investigation about the immunogenetic predisposition to malignant haematological diseases.

查看原文本刊更多论文

单倍体造血干细胞移植中供体-受体配对KIR基因型的频率和分布：西班牙组织相容性和移植免疫学工作组（GETHIT）和西班牙造血移植和细胞治疗组（GETH-TC）的合作研究

与HLA系统相比，关于KIR基因型在单倍体造血干细胞移植（haploi - hsct）中的影响的信息有限。本研究旨在确定接受单倍造血干细胞移植的西班牙血液病患者中KIR基因型的频率。一项研究对113名肿瘤血液病患者及其供体进行了研究，这些患者在西班牙组织相容性和移植免疫学工作组（GETHIT）和西班牙造血移植和细胞治疗小组（GETH-TC）的五个中心接受治疗。采用PCR-rSSO或PCR-SSP进行KIR分型。利用KIR等位基因频率网络数据库对KIR基因型进行鉴定。在献血者中，最常见的KIR基因型是1型（28.3%）、2型（12.4%）和4型（10.6%）。在患者中，基因型1（23.9%）、4（23%）和2（14.2%）最为常见。供者表现为AA型着丝粒（46%）和端粒（59.3%），而患者有较高的AB型着丝粒频率（52.2%）。在BB型中观察到差异(3.5%患者；16.8% （p = 0.002）。AB型KIR基因型最常见（70.8%）；75.2%的患者)。大多数被归类为“中立”（61.9%）；73.5%的患者)。B-content score1最常见(48.7%；33.6%的捐助者)。值得注意的是，最好的分类是罕见的(2.7%的患者；16.8% （p = 0.002）。该研究强调了KIR基因型在单倍hsct患者和供体中的分布，其中基因型1、2和4最为普遍。AB KIR基因型和b含量1分占主导地位。此外，KIR基因型ID可作为未来研究恶性血液病免疫遗传易感性的标准。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

HLA Immunology and Microbiology-Immunology

CiteScore

3.00

自引率

28.80%

发文量

368

期刊介绍： HLA, the journal, publishes articles on various aspects of immunogenetics. These include the immunogenetics of cell surface antigens, the ontogeny and phylogeny of the immune system, the immunogenetics of cell interactions, the functional aspects of cell surface molecules and their natural ligands, and the role of tissue antigens in immune reactions. Additionally, the journal covers experimental and clinical transplantation, the relationships between normal tissue antigens and tumor-associated antigens, the genetic control of immune response and disease susceptibility, and the biochemistry and molecular biology of alloantigens and leukocyte differentiation. Manuscripts on molecules expressed on lymphoid cells, myeloid cells, platelets, and non-lineage-restricted antigens are welcomed. Lastly, the journal focuses on the immunogenetics of histocompatibility antigens in both humans and experimental animals, including their tissue distribution, regulation, and expression in normal and malignant cells, as well as the use of antigens as markers for disease.