Light modulates plant-derived extracellular vesicle properties: a photosensitive-responsive nanodelivery system

IF 4.1 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nanoscale Research Letters Pub Date : 2025-05-21 DOI:10.1186/s11671-025-04266-y

Yu Lu, Wenyu Zhang, Peiyuan Zeng, Yuqian He, Xiaolu Hua, Yan Liu, Jianbo Wu

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引用次数: 0

Abstract

Plant-derived extracellular vesicles (PDEVs) have emerged as innovative nanocarriers for drug delivery, offering advantages such as biocompatibility, stability, and cost-effectiveness. This study explores light-mediated strategies to optimize cargo encapsulation into PDEVs while preserving structural integrity. Leveraging the intrinsic photosensitizing properties of PDEVs, light irradiation (LED) triggered reactive oxygen species (ROS) generation, including superoxide anions and singlet oxygen, which transiently enhanced membrane permeability for controlled drug loading. Using FITC-dextran (70 kDa) as a model cargo, we optimized light-induced loading efficiency, achieving a peak (~ 80%) at 10 min of irradiation. Prolonged exposure (15 min) reduced efficiency (~ 50%), likely due to excessive ROS-induced membrane destabilization. The optimal PDEVs-to-cargo ratio (1:30) ensured maximal loading while maintaining stability over 30 days. Lipid peroxidation analysis further confirmed ROS-induced membrane modifications through malondialdehyde (MDA) accumulation. These findings demonstrate that PLDENs (Pueraria lobate-derived exosomes-like nanovesicles) function as light-responsive nanocarriers, balancing ROS-mediated permeability enhancement with structural integrity. This light-triggered strategy balances permeability modulation and structural integrity, advancing PDEVs as scalable, non-invasive platforms for precision drug delivery and photodynamic applications.

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光调节植物源性细胞外囊泡特性：一种光敏响应纳米递送系统

植物源性细胞外囊泡（PDEVs）已成为一种创新的纳米药物载体，具有生物相容性、稳定性和成本效益等优点。本研究探讨了光介导策略，以优化货物封装到PDEVs，同时保持结构完整性。利用PDEVs固有的光敏特性，光照射（LED）触发活性氧（ROS）的产生，包括超氧阴离子和单线态氧，从而瞬间增强膜的通透性，从而控制药物负载。以fitc -葡聚糖（70 kDa）作为模型货，我们优化了光诱导加载效率，在照射10 min时达到峰值（~ 80%）。长时间暴露（15分钟）降低了效率（~ 50%），可能是由于过量的ros引起的膜不稳定。最佳的pdev -to-cargo比（1:30）确保了最大的装载量，同时在30天内保持稳定。脂质过氧化分析进一步证实了ros通过丙二醛（MDA）积累诱导的膜修饰。这些发现表明plden（葛根衍生的外泌体样纳米囊泡）作为光响应纳米载体，平衡ros介导的通透性增强和结构完整性。这种光触发策略平衡了渗透性调节和结构完整性，使PDEVs成为可扩展的、非侵入性的精确药物输送和光动力学应用平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanoscale Research Letters 工程技术-材料科学：综合

CiteScore

11.30

自引率

0.00%

发文量

110

审稿时长

48 days

期刊介绍： Nanoscale Research Letters (NRL) provides an interdisciplinary forum for communication of scientific and technological advances in the creation and use of objects at the nanometer scale. NRL is the first nanotechnology journal from a major publisher to be published with Open Access.