Fermented brewer's spent grain as a sustainable feed ingredient impacts meagre (Argyrosomus regius) growth, intermediary metabolism, digestive enzymes, oxidative stress, and intestinal histology

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-05-16 DOI:10.1016/j.anifeedsci.2025.116388

T. Estevão-Rodrigues , H. Fernandes , S. Moutinho , A. Couto , I. Belo , C. Castro. , P. Pousão , J.M. Salgado , A. Oliva-Teles , H. Peres

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引用次数: 0

Abstract

It was previously observed that solid-state fermentation (SSF) with Aspergillus ibericus increased brewer's spent grain (BSG) protein content (21 %) while reducing lipid (49 %), cellulose (30 %), hemicellulose (34 %), and lignin (7.3 %) contents. This study evaluated the potential of fermented BSG (BSG-SSF) as a novel feed ingredient for meagre (Argyrosomus regius) juveniles by evaluating growth performance, digestive function, intermediary metabolism, oxidative status, and intestinal histomorphology. Three diets were formulated: a control diet without BSG (15 % fish meal and 66 % plant ingredients) and two diets incorporating 10 % BSG or BSG-SSF, replacing 10 % of the plant ingredients. The diets were tested in triplicate for 10 weeks with meagre averaging 83 g initial weight. The dietary inclusion of BSG reduced fish growth performance and feed and protein utilization efficiency, while feed intake was unaffected. However, including BSG-SSF restored these parameters to the control diet levels. At the end of the trial, no significant differences were observed in whole-body composition, plasma metabolite profile, and intermediary metabolism enzyme activity. Hepatic and intestinal antioxidant enzyme activity and lipid peroxidation also remained similar among diets. The dietary inclusion of BSG and BSG-SSF negatively impacted anterior intestine histomorphology compared to the control. However, the mean histomorphological score in the distal intestine was higher in fish fed BSG than in those fed BSG-SSF, which did not differ from the control. Overall, these findings indicate that BSG-SSF can be included in meagre juvenile diets without compromising growth performance or oxidative status, supporting the sustainable use of agro-industrial by-products in aquafeeds. Future research should focus on evaluating the digestibility of BSG and BSG-SSF, exploring higher inclusion levels of BSG-SSF, and assessing their long-term effects on meagre health and production efficiency.

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发酵啤酒糟作为一种可持续的饲料原料，会影响山羊（Argyrosomus regius）的生长、中间代谢、消化酶、氧化应激和肠道组织学

先前观察到，用伊曲霉进行固态发酵（SSF）提高了啤酒废粒（BSG）蛋白质含量（21 %），同时降低了脂质（49 %）、纤维素（30 %）、半纤维素（34 %）和木质素（7.3 %）含量。本研究通过评价发酵羊sg - ssf的生长性能、消化功能、中间代谢、氧化状态和肠道组织形态学等指标，对发酵羊sg - ssf作为瘦肉仔鱼（Argyrosomus regius）新型饲料原料的潜力进行了评价。配制了三种饲料：不含鱼sg的对照饲料（15 %鱼粉和66 %植物成分）和两种含有10 %鱼sg或BSG- ssf的饲料，替代10 %植物成分。这些饮食分三份进行了为期10周的试验，初始体重平均为83 g。饲料中添加鱼sg降低了鱼的生长性能、饲料和蛋白质利用效率，但采食量不受影响。然而，加入BSG-SSF使这些参数恢复到对照饮食水平。在试验结束时，在全身成分、血浆代谢物谱和中间代谢酶活性方面没有观察到显著差异。肝脏和肠道抗氧化酶活性和脂质过氧化也保持相似。与对照组相比，饲粮中添加BSG和BSG- ssf对前肠组织形态有负面影响。然而，饲喂BSG的鱼远端肠的平均组织形态学评分高于饲喂BSG- ssf的鱼，这与对照组没有差异。总体而言，这些研究结果表明，BSG-SSF可以在不影响生长性能或氧化状态的情况下添加到贫幼鱼饲料中，从而支持在水产饲料中可持续利用农工副产品。未来的研究应侧重于评价牛肉精和牛肉精- ssf的消化率，探索更高的牛肉精- ssf添加水平，并评估其对健康和生产效率的长期影响。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.