{"title":"Interactive effects of nitrogen addition and drought on soil microbial carbon use efficiency in saline–alkaline grasslands of northern China","authors":"Huajie Diao, Jingjing Wang, Yicong Chen, Yangyang Gao, Wenjun Liang, Gaoliang Pang, Jiachen Bian, Jianyu Wang, Jie Hao, Changhui Wang, Xiang Zhao, Kuanhu Dong","doi":"10.1007/s11104-025-07483-7","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Aims</h3><p>Understanding changes in soil microbial carbon use efficiency (CUE) and microbial biomass turnover time are of great significance for increasing ecosystem C sequestration and achieving the strategic goal of C neutrality. However, the response of soil microbial CUE to nitrogen (N) deposition during extreme drought and wetness events in saline–alkaline grasslands remains unclear.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Herein, using an innovative substrate-independent method by incorporating the isotope from H<sub>2</sub><sup>18</sup>O into the DNA of microorganisms, the soil microbial CUE under N addition and precipitation changes (± 50% of natural precipitation) were studied.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>Results showed that the microbial CUE was ranged from 0.29 to 0.59, with an average of 0.46. N addition and precipitation changes alone had no significant effect on soil microbial CUE. N addition combined with drought showed significantly negative influences on the relative changes in soil microbial CUE (decreased by 16.3%), while showed significantly positive influences on microbial biomass turnover time (increased by 96.0%). There was a negative correlation between the soil microbial CUE and microbial biomass turnover time. Microbial CUE was positively correlated with plant biomass, soil water content, and soil bacterial abundance, whereas it was negatively correlated with soil dissolved organic C and soil fungal abundance. Variation partitioning analysis showed that plants, soil environment, soil available nutrients, and soil microbes co-regulated the variation in soil microbial CUE, and the plant biomass, soil fungi, and SWC were the core factors that affected soil microbial CUE.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>Overall, we highlighted that N addition reduces soil C sequestration potential under droughts conditions.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"31 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-025-07483-7","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Aims
Understanding changes in soil microbial carbon use efficiency (CUE) and microbial biomass turnover time are of great significance for increasing ecosystem C sequestration and achieving the strategic goal of C neutrality. However, the response of soil microbial CUE to nitrogen (N) deposition during extreme drought and wetness events in saline–alkaline grasslands remains unclear.
Methods
Herein, using an innovative substrate-independent method by incorporating the isotope from H218O into the DNA of microorganisms, the soil microbial CUE under N addition and precipitation changes (± 50% of natural precipitation) were studied.
Results
Results showed that the microbial CUE was ranged from 0.29 to 0.59, with an average of 0.46. N addition and precipitation changes alone had no significant effect on soil microbial CUE. N addition combined with drought showed significantly negative influences on the relative changes in soil microbial CUE (decreased by 16.3%), while showed significantly positive influences on microbial biomass turnover time (increased by 96.0%). There was a negative correlation between the soil microbial CUE and microbial biomass turnover time. Microbial CUE was positively correlated with plant biomass, soil water content, and soil bacterial abundance, whereas it was negatively correlated with soil dissolved organic C and soil fungal abundance. Variation partitioning analysis showed that plants, soil environment, soil available nutrients, and soil microbes co-regulated the variation in soil microbial CUE, and the plant biomass, soil fungi, and SWC were the core factors that affected soil microbial CUE.
Conclusions
Overall, we highlighted that N addition reduces soil C sequestration potential under droughts conditions.
期刊介绍：
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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