Probabilistic Mapping and Automated Segmentation of Human Brainstem White Matter Bundles.

Abstract

Brainstem white matter bundles are essential conduits for neural signaling involved in modulation of vital functions ranging from homeostasis to human consciousness. Their architecture forms the anatomic basis for brainstem connectomics, subcortical mesoscale circuit models, and deep brain navigation tools. However, their small size and complex morphology compared to cerebral white matter structures makes mapping and segmentation challenging in neuroimaging. This results in a near absence of automated brainstem white matter tracing methods. We leverage diffusion MRI tractography to create BrainStem Bundle Tool (BSBT), which segments eight key white matter bundles in the rostral brainstem. BSBT performs automated segmentation on a custom probabilistic fiber map generated from tractography with a convolutional neural network architecture tailored for detection of small structures. We demonstrate BSBTs robustness across diffusion MRI acquisition protocols through validation on healthy subject in vivo scans and ex vivo scans of brain specimens with corresponding histology. Using BSBT, we reveal distinct brainstem white matter bundle alterations in Alzheimer's disease, Parkinson's disease, and acute traumatic brain injury cohorts through tract-based analysis and classification tasks. Finally, we provide proof-of-principle evidence supporting the prognostic utility of BSBT in a longitudinal analysis of coma recovery. BSBT creates opportunities to automatically map brainstem white matter in large imaging cohorts and investigate its role in a broad spectrum of neurological disorders.