A Matrix-Derived Bioactive Peptide Enhances Epidermal Thickness and Hair Follicle Neogenesis in Grafted Dermal-Epidermal Composites.

IF 3.4 3区 医学 Q2 CELL BIOLOGY
Sandhya Xavier, Jahnabi Roy, Shaowei Li, Peter J Klover, Rajesh L Thangapazham, Ji-An Wang, Donald C Aduba, Sorana Raiciulescu, Leonard C Sperling, Ira M Herman, Thomas N Darling
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Abstract

Cutaneous wounds can be treated using skin substitutes, but they heal with scarring and absence of skin adnexal structures. We previously demonstrated hair follicle neogenesis in dermal-epidermal composites made of neonatal foreskin human keratinocytes and human dermal papilla cells grafted onto nude mice. A challenge to adapting this approach to graft large areas in humans is that dermal papilla cells lose trichogenicity when expanded in vitro. Herein, a peptide derived from a coiled-coil domain of multimerin-1, TSN6, was evaluated for its effects on graft characteristics and hair follicle formation. In a hair follicle reconstitution assay, TSN6 increased the number of hair fibres by 1.8-fold (p value < 0.05). Dermal-epidermal composites, constructed using late-passage human dermal papilla cells and incubated with TSN6 prior to grafting, retained 14 of 14 grafts for 10-12 weeks, whereas scrambled and vehicle groups kept only 9 of 12 and 13 of 16 grafts, respectively. Histological evaluation of skin grafts showed the presence of human hair follicles in 12 of 14 dermal-epidermal composites in the TSN6 group, 3 of 9 in the scrambled group and 6 of 13 in the vehicle group. The median number and interquartile range of hair follicles was 4.5 (1.8, 10.3) for the TSN6 group, 0 (0, 3.5) for the scrambled group and 0 (0, 3.3) for the vehicle group. TSN6 also increased epidermal thickness, showing a thickness of 127 ± 18 μm for the TSN6 group and 70 ± 28 μm and 94 ± 18 μm for the scrambled and vehicle groups, respectively. In summary, TSN6 increases epidermal thickness and promotes hair follicle neogenesis in a skin substitute.

基质来源的生物活性肽促进真皮-表皮复合材料的表皮厚度和毛囊新生。
皮肤伤口可以用皮肤替代品治疗,但它们愈合时留下疤痕,没有皮肤附件结构。我们之前已经证明了由新生包皮、人角质形成细胞和人真皮乳头细胞移植到裸鼠身上的真皮-表皮复合材料可以产生毛囊新生。将这种方法应用于人类大面积移植的一个挑战是,真皮乳头细胞在体外扩增时失去了毛原性。本研究评估了从多聚蛋白-1的卷曲结构域衍生的肽TSN6对移植物特性和毛囊形成的影响。在毛囊重建实验中,TSN6使毛纤维数量增加了1.8倍(p值)
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来源期刊
Wound Repair and Regeneration
Wound Repair and Regeneration 医学-皮肤病学
CiteScore
5.90
自引率
3.40%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Wound Repair and Regeneration provides extensive international coverage of cellular and molecular biology, connective tissue, and biological mediator studies in the field of tissue repair and regeneration and serves a diverse audience of surgeons, plastic surgeons, dermatologists, biochemists, cell biologists, and others. Wound Repair and Regeneration is the official journal of The Wound Healing Society, The European Tissue Repair Society, The Japanese Society for Wound Healing, and The Australian Wound Management Association.
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