Selective serotonin reuptake inhibitors (SSRIs) suppress Na+- dependent Mg2+ efflux in rat ventricular myocytes.

IF 2.6 4区 医学 Q2 PHYSIOLOGY
Michiko Tashiro, Masato Konishi, Hana Inoue, Utako Yokoyama
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引用次数: 0

Abstract

Na+/Mg2+ exchange transport, the Na+ gradient-driven Mg2+ extrusion system, plays a key role in cellular Mg2+ homeostasis. To date, the molecular entity and selective inhibitors of Na+/Mg2+ exchanger have not been fully explored. Intracellular free Mg2+ concentration ([Mg2+]i) was measured in ventricular myocytes acutely isolated from rat hearts. After soaking the cells in high-Mg2+ low-Na+ solution to increase [Mg2+]i, the addition of extracellular Na+ caused a decrease in [Mg2+]i. We analyzed the rate of decrease in [Mg2+]i as Na+/Mg2+ exchange transport activity. The suppression of the rate of decrease in [Mg2+]i caused by sertraline, a selective serotonin reuptake inhibitor (SSRI), was concentration dependent (IC50 8.9 μM) and reversible. Other SSRIs, namely paroxetine and fluvoxamine, were less effective than sertraline. In conclusion, sertraline inhibited Na+/Mg2+ exchange transport more effectively than any previously reported inhibitors of Na+/Mg2+ exchanger. Sertraline could be used as a tool to characterize the functions of Na+/Mg2+ exchanger.

选择性血清素再摄取抑制剂(SSRIs)抑制大鼠心室肌细胞Na+依赖性Mg2+外排。
Na+/Mg2+交换转运是Na+梯度驱动的Mg2+挤压系统,在细胞Mg2+稳态中起关键作用。迄今为止,Na+/Mg2+交换剂的分子实体和选择性抑制剂尚未得到充分的探索。测定急性分离大鼠心肌细胞胞内游离Mg2+浓度([Mg2+]i)。在高Mg2+低Na+溶液中浸泡细胞以增加[Mg2+]i后,加入细胞外Na+使[Mg2+]i降低。我们分析了Na+/Mg2+交换转运活性降低的速率。选择性5 -羟色胺再摄取抑制剂(SSRI)舍曲林对[Mg2+]i下降速率的抑制呈浓度依赖性(IC50为8.9 μM)且可逆。其他SSRIs,即帕罗西汀和氟伏沙明,不如舍曲林有效。综上所述,舍曲林比以往报道的Na+/Mg2+交换剂抑制剂更有效地抑制Na+/Mg2+交换转运。舍曲林可以作为表征Na+/Mg2+交换剂功能的工具。
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来源期刊
CiteScore
4.40
自引率
4.30%
发文量
27
审稿时长
6-12 weeks
期刊介绍: The Journal of Physiological Sciences publishes peer-reviewed original papers, reviews, short communications, technical notes, and letters to the editor, based on the principles and theories of modern physiology and addressed to the international scientific community. All fields of physiology are covered, encompassing molecular, cellular and systems physiology. The emphasis is on human and vertebrate physiology, but comparative papers are also considered. The process of obtaining results must be ethically sound. Fields covered: Adaptation and environment Autonomic nervous function Biophysics Cell sensors and signaling Central nervous system and brain sciences Endocrinology and metabolism Excitable membranes and neural cell physiology Exercise physiology Gastrointestinal and kidney physiology Heart and circulatory physiology Molecular and cellular physiology Muscle physiology Physiome/systems biology Respiration physiology Senses.
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