Michiko Tashiro, Masato Konishi, Hana Inoue, Utako Yokoyama
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引用次数: 0
Abstract
Na+/Mg2+ exchange transport, the Na+ gradient-driven Mg2+ extrusion system, plays a key role in cellular Mg2+ homeostasis. To date, the molecular entity and selective inhibitors of Na+/Mg2+ exchanger have not been fully explored. Intracellular free Mg2+ concentration ([Mg2+]i) was measured in ventricular myocytes acutely isolated from rat hearts. After soaking the cells in high-Mg2+ low-Na+ solution to increase [Mg2+]i, the addition of extracellular Na+ caused a decrease in [Mg2+]i. We analyzed the rate of decrease in [Mg2+]i as Na+/Mg2+ exchange transport activity. The suppression of the rate of decrease in [Mg2+]i caused by sertraline, a selective serotonin reuptake inhibitor (SSRI), was concentration dependent (IC50 8.9 μM) and reversible. Other SSRIs, namely paroxetine and fluvoxamine, were less effective than sertraline. In conclusion, sertraline inhibited Na+/Mg2+ exchange transport more effectively than any previously reported inhibitors of Na+/Mg2+ exchanger. Sertraline could be used as a tool to characterize the functions of Na+/Mg2+ exchanger.
期刊介绍:
The Journal of Physiological Sciences publishes peer-reviewed original papers, reviews, short communications, technical notes, and letters to the editor, based on the principles and theories of modern physiology and addressed to the international scientific community. All fields of physiology are covered, encompassing molecular, cellular and systems physiology. The emphasis is on human and vertebrate physiology, but comparative papers are also considered. The process of obtaining results must be ethically sound.
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