Characterizing the gut microbiota composition in Taiwanese hypertensive patients using 16S rRNA sequencing analysis.

IF 3.2 3区 医学 Q1 MEDICINE, GENERAL & INTERNAL
International Journal of Medical Sciences Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI:10.7150/ijms.109340
Ming-Shan Chen, Shin-Kuang Jiang, Zhi-Yong Chong, Jou-Wei Chiang, Yan-Min Chen, Hsin-Yu Huang, Jui-Chieh Chen
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引用次数: 0

Abstract

Hypertension (HTN) is a significant risk factor for cardiovascular and cerebrovascular diseases. Accumulating evidence suggests a close relationship between HTN and alterations in the gut microbiota composition and abundance. We recruited 23 HTN patients and 17 controls matched for demographic characteristics. DNA extracted from fecal samples of patients was subjected to Illumina MiSeq sequencing, targeting the V3-V4 region of the bacterial 16S rRNA gene for analysis. We compared the diversity and composition of gut microbiota between the two groups. The α-diversity of gut microbiota in HTN patients was similar to that in the control group. β-diversity analysis showed slight differences in microbial composition between the HTN and control groups. We used Welch's t-test to evaluate the significant difference in the bacterial composition of the top 20 ASVs between the HTN group and the control group, and the results showed that Tyzzerella was significantly increased, while Faecalibacterium was significantly decreased in the HTN group. We performed PCR using Faecalibacterium-specific primers and analyzed their levels through agarose gel electrophoresis, confirming the reduced abundance of Faecalibacterium in the HTN group. In addition, Tax4Fun2 analysis was employed to examine differences in microbial functionality between the HTN group and the control group. In conclusion, we studied the fecal microbiota of HTN population in Taiwan through 16S rRNA gene sequencing, and found that Faecalibacterium has a lower abundance in HTN patients. This unique alteration in gut microbiota may provide insights into the pathogenesis of HTN and aid in the development of novel biomarkers and therapeutic targets.

利用16S rRNA测序分析台湾高血压患者肠道菌群组成。
高血压(HTN)是心脑血管疾病的重要危险因素。越来越多的证据表明,HTN与肠道微生物群组成和丰度的变化密切相关。我们招募了23名HTN患者和17名符合人口统计学特征的对照组。从患者粪便样本中提取DNA进行Illumina MiSeq测序,针对细菌16S rRNA基因的V3-V4区域进行分析。我们比较了两组之间肠道菌群的多样性和组成。HTN患者肠道菌群α-多样性与对照组相似。β-多样性分析显示,HTN与对照组之间的微生物组成略有差异。我们采用Welch’st检验评价HTN组与对照组前20名asv细菌组成差异的显著性,结果显示HTN组Tyzzerella显著增加,Faecalibacterium显著减少。我们使用Faecalibacterium特异性引物进行PCR,并通过琼脂糖凝胶电泳分析其水平,证实HTN组Faecalibacterium的丰度降低。此外,采用Tax4Fun2分析来检查HTN组与对照组之间微生物功能的差异。综上所述,我们通过16S rRNA基因测序研究了台湾HTN人群的粪便微生物群,发现Faecalibacterium在HTN患者中丰度较低。肠道微生物群的这种独特变化可能为HTN的发病机制提供新的见解,并有助于开发新的生物标志物和治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Medical Sciences
International Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
7.20
自引率
0.00%
发文量
185
审稿时长
2.7 months
期刊介绍: Original research papers, reviews, and short research communications in any medical related area can be submitted to the Journal on the understanding that the work has not been published previously in whole or part and is not under consideration for publication elsewhere. Manuscripts in basic science and clinical medicine are both considered. There is no restriction on the length of research papers and reviews, although authors are encouraged to be concise. Short research communication is limited to be under 2500 words.
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