Transcutaneous auricular vaguas nerve stimulation promotes adipose tissue browning and mitochondrial integrity by regulating BCAA metabolism in obese rats: an experimental study.

IF 12.5 2区医学 Q1 SURGERY

International journal of surgery Pub Date : 2025-05-16 DOI:10.1097/JS9.0000000000002451

Chen Xin, Shaoyuan Li, Bowen Feng, Lan Sun, Yifei Wang, Jinling Zhang, Yuzhengheng Zhang, Ningyi Zou, Qing Zhou, Peijing Rong

{"title":"Transcutaneous auricular vaguas nerve stimulation promotes adipose tissue browning and mitochondrial integrity by regulating BCAA metabolism in obese rats: an experimental study.","authors":"Chen Xin, Shaoyuan Li, Bowen Feng, Lan Sun, Yifei Wang, Jinling Zhang, Yuzhengheng Zhang, Ningyi Zou, Qing Zhou, Peijing Rong","doi":"10.1097/JS9.0000000000002451","DOIUrl":null,"url":null,"abstract":"<p><strong>Abstract: </strong>Transcutaneous auricular vagus nerve stimulation (taVNS) may exert its anti-obesity effects by promoting the browning of adipose tissue with unclear mechanisms. Modulation of branched-chain amino acid (BCAA) metabolism in adipose tissue may play a key role in this process. In this study, six weeks of taVNS significantly reduced body weight gain, body mass index, and the accumulation of inguinal and epididymal white adipose tissue in high-fat diet (HFD)-induced obese rats, with effects comparable to those of orlistat. Notably, taVNS increased both the content and thermogenic activity of interscapular brown adipose tissue (BAT) in obese rats. Infrared thermal imaging confirmed increased BAT thermogenesis, while transmission electron microscopy indicated improved mitochondrial morphology and function. Molecular analysis revealed that taVNS upregulated uncoupling protein 1 (UCP1), the transporter protein solute carrier family 25 member 44 (SLC25A44), and protein phosphatase Mg 2 + /Mn 2 + dependent 1 K (PPM1K) expression while downregulating branched-chain α-ketoacid dehydrogenase kinase (BCKDK), thereby promoting BCAA metabolism and adipose tissue browning. We conclude that the anti-obesity effects of taVNS are closely related to BCAA metabolism in BAT. These findings highlight taVNS as a promising approach for obesity management, though further studies are needed to clarify the neurobiological pathways involved and advance taVNS technology.</p>","PeriodicalId":14401,"journal":{"name":"International journal of surgery","volume":" ","pages":""},"PeriodicalIF":12.5000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/JS9.0000000000002451","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SURGERY","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract: Transcutaneous auricular vagus nerve stimulation (taVNS) may exert its anti-obesity effects by promoting the browning of adipose tissue with unclear mechanisms. Modulation of branched-chain amino acid (BCAA) metabolism in adipose tissue may play a key role in this process. In this study, six weeks of taVNS significantly reduced body weight gain, body mass index, and the accumulation of inguinal and epididymal white adipose tissue in high-fat diet (HFD)-induced obese rats, with effects comparable to those of orlistat. Notably, taVNS increased both the content and thermogenic activity of interscapular brown adipose tissue (BAT) in obese rats. Infrared thermal imaging confirmed increased BAT thermogenesis, while transmission electron microscopy indicated improved mitochondrial morphology and function. Molecular analysis revealed that taVNS upregulated uncoupling protein 1 (UCP1), the transporter protein solute carrier family 25 member 44 (SLC25A44), and protein phosphatase Mg 2 + /Mn 2 + dependent 1 K (PPM1K) expression while downregulating branched-chain α-ketoacid dehydrogenase kinase (BCKDK), thereby promoting BCAA metabolism and adipose tissue browning. We conclude that the anti-obesity effects of taVNS are closely related to BCAA metabolism in BAT. These findings highlight taVNS as a promising approach for obesity management, though further studies are needed to clarify the neurobiological pathways involved and advance taVNS technology.

查看原文本刊更多论文

经皮耳迷走神经刺激通过调节肥胖大鼠BCAA代谢促进脂肪组织褐变和线粒体完整性：一项实验研究。

摘要：经皮耳迷走神经刺激（taVNS）可能通过促进脂肪组织褐化发挥其抗肥胖作用，但机制尚不清楚。脂肪组织中支链氨基酸（BCAA）代谢的调节可能在这一过程中起关键作用。在这项研究中，6周的taVNS显著降低了高脂肪饮食（HFD）诱导的肥胖大鼠的体重增加、体重指数以及腹股沟和附睾白色脂肪组织的积累，其效果与奥利司他相当。值得注意的是，taVNS增加了肥胖大鼠肩胛间棕色脂肪组织（BAT）的含量和产热活性。红外热成像证实BAT产热增加，透射电镜显示线粒体形态和功能改善。分子分析显示，taVNS上调解偶联蛋白1 （UCP1）、转运蛋白溶质载体家族25成员44 （SLC25A44）和蛋白磷酸酶Mg 2 + /Mn 2 +依赖性1k （PPM1K）表达，下调支链α-酮酸脱氢酶激酶（BCKDK），从而促进BCAA代谢和脂肪组织褐化。我们认为，taVNS的抗肥胖作用与BAT中的BCAA代谢密切相关。这些发现强调了taVNS作为一种有希望的肥胖治疗方法，尽管需要进一步的研究来阐明所涉及的神经生物学途径并推进taVNS技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International journal of surgery SURGERY-

CiteScore

17.70

自引率

3.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The International Journal of Surgery (IJS) has a broad scope, encompassing all surgical specialties. Its primary objective is to facilitate the exchange of crucial ideas and lines of thought between and across these specialties.By doing so, the journal aims to counter the growing trend of increasing sub-specialization, which can result in "tunnel-vision" and the isolation of significant surgical advancements within specific specialties.