Impact of Storage Conditions on the Stability of Urinary Biomarkers.

IF 0.7 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Clinical laboratory Pub Date : 2025-05-01 DOI:10.7754/Clin.Lab.2024.240939

Chanyuan Zhang, Tiebing Liu, Shanshan Xu, Lijiao Xia, Chunyang Zhu, Haiyuan Zhao, Guowei Jia, Hongjinwen Gao, Xuejing Wang

{"title":"Impact of Storage Conditions on the Stability of Urinary Biomarkers.","authors":"Chanyuan Zhang, Tiebing Liu, Shanshan Xu, Lijiao Xia, Chunyang Zhu, Haiyuan Zhao, Guowei Jia, Hongjinwen Gao, Xuejing Wang","doi":"10.7754/Clin.Lab.2024.240939","DOIUrl":null,"url":null,"abstract":"Background: This study aimed to assess the influence of different storage conditions and pH levels on biomarkers in urine.Methods: Urine samples were collected from 20 healthy individuals and 20 patients with kidney disease. The stability of nine proteins with normal and pathological concentrations was investigated under 4 types of storage conditions: 25°C for 8 hours, 4°C for 7 days, -20°C for 12 weeks, and -80°C for 12 months. Ten pH values (4.0 to 8.5) were also tested. Nine biomarkers included U-α1MG, U-Alb, U-Trf, U-IgG, U-NAG, U-NGAL, U-RBP, U-CysC, and U-TP, mostly measured in the laboratory.Results: U-Alb, U-α1MG, U-Trf, U-TP, and U-CysC were relatively stable across four storage conditions. However, the stability of other biomarkers may be impacted by the storage conditions. For instance, U-IgG was unstable at -20°C after eight weeks and -80°C after one month. U-NAG was unstable at 4°C after four days and -20°C for 12 weeks. U-RBP was unstable at -80°C after five months and showed a significant upward trend at -20°C within 12 weeks in the pathological levels. Regarding the impact of the pH levels, the bias of U-α1MG and U-CysC did not exceed ± 10% across the pH range of 5.0 to 7.5. The bias of another 5 biomarkers (U-Alb, U-Trf, U-IgG, U-NGAL, and U-TP) was less than -10% at pH 5.0 to 6.0. U-NAG and U-RBP were unstable, with bias exceeding -10%.Conclusions: U-NAG and U-RBP should not be stored at -20°C. U-IgG could only be stable for a short period under -20°C and -80°C. Urinary pH should be monitored and adjusted if necessary.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 5","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical laboratory","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.7754/Clin.Lab.2024.240939","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: This study aimed to assess the influence of different storage conditions and pH levels on biomarkers in urine.

Methods: Urine samples were collected from 20 healthy individuals and 20 patients with kidney disease. The stability of nine proteins with normal and pathological concentrations was investigated under 4 types of storage conditions: 25°C for 8 hours, 4°C for 7 days, -20°C for 12 weeks, and -80°C for 12 months. Ten pH values (4.0 to 8.5) were also tested. Nine biomarkers included U-α1MG, U-Alb, U-Trf, U-IgG, U-NAG, U-NGAL, U-RBP, U-CysC, and U-TP, mostly measured in the laboratory.

Results: U-Alb, U-α1MG, U-Trf, U-TP, and U-CysC were relatively stable across four storage conditions. However, the stability of other biomarkers may be impacted by the storage conditions. For instance, U-IgG was unstable at -20°C after eight weeks and -80°C after one month. U-NAG was unstable at 4°C after four days and -20°C for 12 weeks. U-RBP was unstable at -80°C after five months and showed a significant upward trend at -20°C within 12 weeks in the pathological levels. Regarding the impact of the pH levels, the bias of U-α1MG and U-CysC did not exceed ± 10% across the pH range of 5.0 to 7.5. The bias of another 5 biomarkers (U-Alb, U-Trf, U-IgG, U-NGAL, and U-TP) was less than -10% at pH 5.0 to 6.0. U-NAG and U-RBP were unstable, with bias exceeding -10%.

Conclusions: U-NAG and U-RBP should not be stored at -20°C. U-IgG could only be stable for a short period under -20°C and -80°C. Urinary pH should be monitored and adjusted if necessary.

查看原文本刊更多论文

储存条件对尿液生物标志物稳定性的影响。

背景：本研究旨在评估不同储存条件和pH水平对尿液中生物标志物的影响。方法：采集20例健康人及20例肾脏疾病患者尿液标本。在25°C保存8小时、4°C保存7天、-20°C保存12周、-80°C保存12个月4种不同的保存条件下，研究了9种正常和病理浓度蛋白的稳定性。还测试了10个pH值（4.0 ~ 8.5）。9种生物标志物包括U-α1MG、U- alb、U- trf、U- igg、U- nag、U- ngal、U- rbp、U- cysc和U- tp，主要在实验室检测。结果：U- alb、U-α1MG、U- trf、U- tp和U- cysc在4种贮藏条件下相对稳定。然而，其他生物标志物的稳定性可能会受到储存条件的影响。例如，U-IgG在-20℃下8周不稳定，在-80℃下1个月不稳定。4天后，U-NAG在4°C下不稳定，在-20°C下持续12周。U-RBP在-80℃下5个月后不稳定，在-20℃下12周内病理水平呈明显上升趋势。在pH值5.0 ~ 7.5范围内，U-α1MG和U- cysc的偏置不超过±10%。另外5种生物标志物（U-Alb、U-Trf、U-IgG、U-NGAL和U-TP）在pH 5.0 ~ 6.0时的偏差小于-10%。U-NAG和U-RBP不稳定，偏差超过-10%。结论：U-NAG和U-RBP不宜在-20℃保存。在-20℃和-80℃条件下，U-IgG只能在短时间内保持稳定。监测尿液pH值，必要时进行调整。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.