Negative Impact of Caloric Restriction on Atherosclerosis in Young ApoE/LDLr ^-/- Mice.

IF 2 Q3 CELL BIOLOGY

Cellular Physiology and Biochemistry Pub Date : 2025-05-12 DOI:10.33594/000000775

Renata B Kostogrys, Magdalena Franczyk-Żarów, Artur Gurgul, Igor Jasielczuk, Anna Żaczek, Magdalena Hubalewska-Mazgaj, Sabina Lichołai, Angelika Manterys, Iwona Wybrańska

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引用次数: 0

Abstract

Background/aims: Caloric restriction (CR) has proven to be the most effective dietary intervention for reducing cardiovascular disease (CVD) associated with obesity. Depending on the age of the mice the effect of caloric restriction was diverse. Therefore, the effect of CR on the development of atherosclerosis in young and adult ApoE/LDLr^-/- mice was evaluated.

Methods: Eight-week-old and 20-week-old male mice received a control diet. Young mice were fed for eight weeks, whereas adult mice for 5 weeks. To assess whether individual housing influenced the tested parameters, control animals were housed in colony cages (AL) or individually (stressAL; sAL) and fed ad libitum. Individually housed caloric restriction (CR) mice received a 30% less diet compared to AL group.

Results: The body weight of CR mice was significantly lower compared to the AL and sAL groups. TCh and LDL levels were significantly increased in young CR mice. No differences in adult animals were observed. TAG levels significantly decreased in both young and adult CR mice. CR induced atherosclerosis in young mice. The FMO3 gene was upregulated in young animals. Microbiota composition changed. At the genus level, compared to the control, CR group exhibited a higher relative abundance of the Enterococcus, Clostridium_sensu_stricto_1 , Rikenella and a lower relative abundance of the CAG_352 (P< 0.05) genera.

Conclusion: Caloric restriction exacerbated atherosclerosis in young ApoE/LDLr^-/- mice.

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热量限制对年轻ApoE/LDLr -/-小鼠动脉粥样硬化的负面影响。

背景/目的：热量限制（CR）已被证明是减少与肥胖相关的心血管疾病（CVD）最有效的饮食干预措施。根据老鼠的年龄，热量限制的效果是不同的。因此，我们评估了CR对年轻和成年ApoE/LDLr-/-小鼠动脉粥样硬化发展的影响。方法：8周龄和20周龄雄性小鼠给予对照饮食。幼鼠饲喂8周，成年鼠饲喂5周。为了评估单独饲养是否影响测试参数，对照动物被饲养在群体笼（AL）或单独（应激笼）中；（sAL），可以随意喂食。单独饲养的热量限制（CR）小鼠比AL组少吃30%的食物。结果：与AL和sAL组相比，CR组小鼠的体重明显降低。年轻CR小鼠的TCh和LDL水平显著升高。在成年动物中没有观察到差异。在年轻和成年CR小鼠中，TAG水平显著降低。CR诱导幼鼠动脉粥样硬化。FMO3基因在幼龄动物中表达上调。微生物群组成改变。在属水平上，CR组Enterococcus、Clostridium_sensu_stricto_1和Rikenella的相对丰度高于对照组（P< 0.05）， CAG_352的相对丰度低于对照组（P< 0.05）。结论：热量限制加重了年轻ApoE/LDLr-/-小鼠的动脉粥样硬化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular Physiology and Biochemistry 生物-生理学

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.