New Nucleoside Derivatives for Hybridization-Assisted Catalysis of Site-Selective Acetylation of 2'-OH of RNA.

IF 1.5 4区 医学 Q4 CHEMISTRY, MEDICINAL
Hayate Takasaki, Kentaro Kitazaki, Yurie Hadano, Hirotaka Murase, Jeongsu Lee, Yosuke Taniguchi, Shigeki Sasaki
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引用次数: 0

Abstract

New nucleoside derivatives containing the imidazole (Imd), pyridine or pyrimidine catalytic group were designed for site-specific acetylation of 2'-OH of the RNA ribose moiety. When the RNA substrate was acetylated in the presence of acetic anhydride under alkaline conditions, Probe (Imd) containing the imidazole catalytic group acetylated with a high selectivity to the 2'-OH of the uridine opposite the catalytic nucleotide. Probe (Py-4N) containing the pyridine group showed a higher catalytic activity under neutral conditions with a high selectivity for the 2'-OH group of the 5' side of the uridine opposite the catalytic nucleotide in about 80% modification yield within 10 min. This study has shown that the oligodeoxynucleotide incorporating the new nucleotide derivative with the catalytic group can be a useful tool for site-selective acetylation of RNA 2'-OH.

杂交辅助催化RNA 2'-OH位点选择性乙酰化的新核苷衍生物。
设计了含有咪唑(Imd)、吡啶或嘧啶催化基团的新核苷衍生物,用于RNA核糖片段2'-OH的位点特异性乙酰化。当RNA底物在碱性条件下乙酸酐存在下乙酰化时,含有咪唑催化基团的探针(Imd)对催化核苷酸对面尿苷的2'-OH具有高选择性乙酰化。含有吡啶基团的探针(Py-4N)在中性条件下表现出较高的催化活性,对与催化核苷酸相对的尿嘧啶5‘侧的2’-OH基团具有较高的选择性,在10分钟内修饰率约为80%。本研究表明,含有新核苷酸衍生物和催化基团的寡脱氧核苷酸可以成为RNA 2'-OH位点选择性乙酰化的有用工具。
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来源期刊
CiteScore
3.20
自引率
5.90%
发文量
132
审稿时长
1.7 months
期刊介绍: The CPB covers various chemical topics in the pharmaceutical and health sciences fields dealing with biologically active compounds, natural products, and medicines, while BPB deals with a wide range of biological topics in the pharmaceutical and health sciences fields including scientific research from basic to clinical studies. For details of their respective scopes, please refer to the submission topic categories below. Topics: Organic chemistry In silico science Inorganic chemistry Pharmacognosy Health statistics Forensic science Biochemistry Pharmacology Pharmaceutical care and science Medicinal chemistry Analytical chemistry Physical pharmacy Natural product chemistry Toxicology Environmental science Molecular and cellular biology Biopharmacy and pharmacokinetics Pharmaceutical education Chemical biology Physical chemistry Pharmaceutical engineering Epidemiology Hygiene Regulatory science Immunology and microbiology Clinical pharmacy Miscellaneous.
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