Sweeteners affect biofilm formation and virulence gene expression in Pseudomonas aeruginosa PAO1.

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2025-05-01 Epub Date: 2025-05-19 DOI:10.1080/08927014.2025.2504023

Tamara Carević Milićević, Katarina Novović, Biljana Nikolić, Dejan Stojković, Vuk Maksimović, Dragica Milosavljević, Marija Ivanov

{"title":"Sweeteners affect biofilm formation and virulence gene expression in Pseudomonas aeruginosa PAO1.","authors":"Tamara Carević Milićević, Katarina Novović, Biljana Nikolić, Dejan Stojković, Vuk Maksimović, Dragica Milosavljević, Marija Ivanov","doi":"10.1080/08927014.2025.2504023","DOIUrl":null,"url":null,"abstract":"Pseudomonas aeruginosa is an opportunistic pathogen able to form biofilms, contributing to its virulence. With the increasing use of sweeteners in various foods, understanding their influence on bacterial behavior is critical. This study investigated the virulence of P. aeruginosa PAO1 exposed to sweeteners (erythritol, stevia, fructose, coconut sugar, cane sugar, demerara). Sweeteners didn't affect growth rates. Erythritol stimulated biofilm (100 µg/mL, 159.98% formation), while 10 µg/mL of coconut sugar, cane sugar, and demerara promoted lower levels (∼70% formation). Erythritol stimulated exopolysaccharides production but reduced biofilm eDNA. Stevia, fructose, and coconut sugar increased the expression of lasI, lasR, rhlI, rhlR, pqsA, mvfR, and pvdF. HPLC analysis confirmed sucrose as the major sugar in demerara, coconut and cane sugar. Erythritol stimulated biofilm and some virulence genes expression, while other sweeteners' effects varied. Cane sugar was a biofilm inhibitor with a limited gene expression effect. The sweeteners' impact on microorganisms is diverse and should be further investigated.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"512-522"},"PeriodicalIF":2.6000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofouling","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/08927014.2025.2504023","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/19 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen able to form biofilms, contributing to its virulence. With the increasing use of sweeteners in various foods, understanding their influence on bacterial behavior is critical. This study investigated the virulence of P. aeruginosa PAO1 exposed to sweeteners (erythritol, stevia, fructose, coconut sugar, cane sugar, demerara). Sweeteners didn't affect growth rates. Erythritol stimulated biofilm (100 µg/mL, 159.98% formation), while 10 µg/mL of coconut sugar, cane sugar, and demerara promoted lower levels (∼70% formation). Erythritol stimulated exopolysaccharides production but reduced biofilm eDNA. Stevia, fructose, and coconut sugar increased the expression of lasI, lasR, rhlI, rhlR, pqsA, mvfR, and pvdF. HPLC analysis confirmed sucrose as the major sugar in demerara, coconut and cane sugar. Erythritol stimulated biofilm and some virulence genes expression, while other sweeteners' effects varied. Cane sugar was a biofilm inhibitor with a limited gene expression effect. The sweeteners' impact on microorganisms is diverse and should be further investigated.

查看原文本刊更多论文

甜味剂影响铜绿假单胞菌PAO1的生物膜形成和毒力基因表达。

铜绿假单胞菌是一种机会性病原体，能够形成生物膜，有助于其毒力。随着甜味剂在各种食品中的使用越来越多，了解它们对细菌行为的影响至关重要。本研究研究了P. aeruginosa PAO1暴露于甜味剂（赤藓糖醇、甜菊糖、果糖、椰子糖、蔗糖、枸橼酸）的毒力。甜味剂不会影响生长速率。赤藓糖醇刺激生物膜（100µg/mL， 159.98%的形成），而10µg/mL的椰子糖、蔗糖和豆芽糖促进较低水平的生物膜（约70%的形成）。赤藓糖醇刺激胞外多糖的产生，但减少了生物膜eDNA。甜菊糖、果糖和椰子糖增加了lasI、lasR、rhlI、rhlR、pqsA、mvfR和pvdF的表达。高效液相色谱分析证实，蔗糖是豆糖、椰子糖和蔗糖中的主要糖。赤藓糖醇刺激了生物膜和一些毒力基因的表达，而其他甜味剂的作用各不相同。蔗糖是一种生物膜抑制剂，具有有限的基因表达作用。甜味剂对微生物的影响是多种多样的，应该进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.