Biofunctionalization of electrospun silk scaffolds with perlecan for vascular tissue engineering.

IF 5.8 3区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Shouyuan Jiang, Anyu Zhang, Behnam Akhavan, John Whitelock, Marcela M Bilek, Steven G Wise, Megan S Lord, Jelena Rnjak-Kovacina
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引用次数: 0

Abstract

Electrospun silk fibroin scaffolds have garnered significant interest in vascular tissue engineering due to their biocompatibility, mechanical strength, and tunable degradation. However, their lack of intrinsic cell-binding domains limits endothelialization, a critical factor for vascular graft success. This study explores the biofunctionalization of electrospun silk scaffolds with recombinant perlecan domain V (rDV) using plasma immersion ion implantation (PIII) treatment, a surface modification method enabling robust covalent immobilization without the use of reagents. The biofunctionalized scaffolds enhanced endothelial cell adhesion, proliferation, and retention under physiological flow conditions while inhibiting smooth muscle cell proliferation. Additionally, the functionalized scaffolds demonstrated angiogenic potential in vivo. These findings underscore the potential of rDV-functionalized silk scaffolds as a promising candidate for small-diameter vascular grafts, addressing key challenges of endothelialization and vascular cell modulation in clinical applications.

电纺丝支架血管组织工程生物功能化研究。
电纺丝蛋白支架由于其生物相容性、机械强度和可调节降解性而在血管组织工程中引起了极大的兴趣。然而,它们缺乏内在的细胞结合结构域,限制了内皮化,这是血管移植成功的关键因素。本研究探讨了利用等离子体浸泡离子注入(PIII)处理重组perlecan结构域V (rDV)的电纺丝支架的生物功能化,这是一种无需使用试剂即可实现强大共价固定的表面修饰方法。生物功能支架增强了内皮细胞在生理流动条件下的粘附、增殖和保留,同时抑制了平滑肌细胞的增殖。此外,功能化支架在体内表现出血管生成的潜力。这些发现强调了rdv功能化丝支架作为小直径血管移植物的潜力,解决了内皮化和血管细胞调节在临床应用中的关键挑战。
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来源期刊
Biomaterials Science
Biomaterials Science MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
11.50
自引率
4.50%
发文量
556
期刊介绍: Biomaterials Science is an international high impact journal exploring the science of biomaterials and their translation towards clinical use. Its scope encompasses new concepts in biomaterials design, studies into the interaction of biomaterials with the body, and the use of materials to answer fundamental biological questions.
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