Unraveling the Anti-Tumor Effects and Molecular Mechanisms of Hairyvein Agrimonia Herb in Gastric Cancer Through Network Pharmacology and Experimental Validation

IF 1.5 Q4 ONCOLOGY

Cancer reports Pub Date : 2025-05-20 DOI:10.1002/cnr2.70169

Hequn He, Xiaohui Jin, Xiaoyun Ding, Haizhong Jiang, Xuguang Wang, Yi Chen, Jiyun Zhu

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引用次数: 0

Abstract

Background

Stomach cancer has become one of the most common types of cancer, with its mortality rate ranking third in the world. Currently, the main treatments for gastric cancer are surgery, radiation therapy, and chemotherapy. Although current treatments can effectively prevent postoperative metastasis and recurrence of gastric cancer, they may also bring various adverse reactions in the gastrointestinal tract and side effects such as bone marrow suppression. Years of research have confirmed that traditional Chinese medicine treatment for gastric and other cancers has distinct characteristics and advantages. Combined treatment can increase the tumor inhibition rate, reduce the side effects of radiation and chemotherapy, improve patients' quality of life, and prolong the survival prognosis.

Aims

This study explores the anti-tumor effect and specific molecular mechanism of Hairyvein Agrimonia Herb on gastric cancer.

Methods and Results

The combination of network pharmacology technology and various experimental techniques, including in vitro cell verification, was carried out throughout the whole study. The results show that five active components in the Hairyvein Agrimonia Herb can act on 160 carcinogenic targets in gastric cancer. String correlation analysis, Cytoscape network topology analysis, core target screening, protein molecule docking, immunohistochemical expression levels, and survival immune correlation analysis revealed that the carcinogenic genes JUN, HIF1A, and PTGS2 may be the primary drug targets for Hairyvein Agrimonia Herb in treating gastric cancer. The active component quercetin shows the best inhibitory effect on the docking of the PTGS2 protein. Furthermore, the prognostic models constructed by the carcinogenic genes JUN, HIF1A, and PTGS2 are significantly correlated with the survival time of gastric cancer patients.

Conclusion

This study provides a new ethical basis and research direction for understanding the mechanism of action of Hairyvein Agrimonia Herb in treating gastric cancer.

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通过网络药理学和实验验证揭示毛缕草对胃癌的抗肿瘤作用及其分子机制

胃癌已成为最常见的癌症类型之一，其死亡率居世界第三位。目前，胃癌的主要治疗方法有手术、放疗和化疗。目前的治疗方法虽然能有效预防胃癌术后转移和复发，但也可能带来胃肠道的各种不良反应和骨髓抑制等副作用。多年的研究证实，中医药治疗胃癌和其他癌症具有明显的特点和优势。联合治疗可提高肿瘤抑制率，减少放化疗副作用，改善患者生活质量，延长生存预后。目的探讨毛缕草对胃癌的抗肿瘤作用及其特异性分子机制。方法与结果将网络药理学技术与包括体外细胞验证在内的多种实验技术相结合，贯穿整个研究过程。结果表明，海参中5种有效成分可作用于胃癌的160个致癌靶点。串相关分析、Cytoscape网络拓扑分析、核心靶点筛选、蛋白分子对接、免疫组化表达水平及生存免疫相关分析显示，致癌基因JUN、HIF1A、PTGS2可能是海丝草治疗胃癌的主要药物靶点。其中活性成分槲皮素对PTGS2蛋白对接的抑制效果最好。此外，由致癌基因JUN、HIF1A和PTGS2构建的预后模型与胃癌患者的生存时间有显著相关性。结论本研究为了解海麦草治疗胃癌的作用机制提供了新的伦理依据和研究方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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