Optimization of laccase production by Pleurotus pulmonarius through solid substrate fermentation of tender coconut fiber: enhanced laccase production and biomass delignification

IF 3.5 4区 工程技术 Q3 ENERGY & FUELS
Geethu Suresh, Ragunathan R, Jesteena Johney
{"title":"Optimization of laccase production by Pleurotus pulmonarius through solid substrate fermentation of tender coconut fiber: enhanced laccase production and biomass delignification","authors":"Geethu Suresh,&nbsp;Ragunathan R,&nbsp;Jesteena Johney","doi":"10.1007/s13399-024-06263-0","DOIUrl":null,"url":null,"abstract":"<div><p>Tender coconut fiber, an abundant agro-waste, requires chemical or biological pretreatment to enhance its potential for value-added applications due to its complex lignocellulosic composition. Pretreatment of tender coconut fiber with laccase-producing white rot fungi presents a sustainable strategy for effective delignification and waste management. This study investigated the biological pretreatment of tender coconut fiber using the white rot fungus, <i>Pleurotus pulmonarius</i>, for delignification through enhanced laccase production via solid-state fermentation. This research involved exploring the influence of different factors on laccase production and understanding the relationships between these factors through response surface methodology (RSM) to obtain the maximum laccase production. A central composite design was employed to optimize the process parameters, including pH, temperature, incubation time, and concentration of corn steep liquor (CSL). The optimized process parameters were a pH of 5.6, a temperature of 27 °C, an incubation time of 21 days, and a CSL concentration of 3%. This optimization resulted in a remarkable increase in enzyme activity, reaching 663.76 ± 10.67 IU/ml, indicating a substantial 13.16-fold increase after the optimization process. Scanning electron microscopy (SEM) and FTIR analysis were performed to observe the changes in the surface structures of the raw and delignified fiber. The enhanced laccase activity suggests the potential for tender coconut fiber to be used to produce laccase enzymes, which has not been explored before. This approach offers a solution for managing tender coconut fiber and unlocks the potential for valorization due to the enhanced properties of the modified fiber.</p></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 9","pages":"13769 - 13781"},"PeriodicalIF":3.5000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomass Conversion and Biorefinery","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13399-024-06263-0","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENERGY & FUELS","Score":null,"Total":0}
引用次数: 0

Abstract

Tender coconut fiber, an abundant agro-waste, requires chemical or biological pretreatment to enhance its potential for value-added applications due to its complex lignocellulosic composition. Pretreatment of tender coconut fiber with laccase-producing white rot fungi presents a sustainable strategy for effective delignification and waste management. This study investigated the biological pretreatment of tender coconut fiber using the white rot fungus, Pleurotus pulmonarius, for delignification through enhanced laccase production via solid-state fermentation. This research involved exploring the influence of different factors on laccase production and understanding the relationships between these factors through response surface methodology (RSM) to obtain the maximum laccase production. A central composite design was employed to optimize the process parameters, including pH, temperature, incubation time, and concentration of corn steep liquor (CSL). The optimized process parameters were a pH of 5.6, a temperature of 27 °C, an incubation time of 21 days, and a CSL concentration of 3%. This optimization resulted in a remarkable increase in enzyme activity, reaching 663.76 ± 10.67 IU/ml, indicating a substantial 13.16-fold increase after the optimization process. Scanning electron microscopy (SEM) and FTIR analysis were performed to observe the changes in the surface structures of the raw and delignified fiber. The enhanced laccase activity suggests the potential for tender coconut fiber to be used to produce laccase enzymes, which has not been explored before. This approach offers a solution for managing tender coconut fiber and unlocks the potential for valorization due to the enhanced properties of the modified fiber.

嫩椰子纤维固体底物发酵肺侧耳菌产漆酶的优化:提高漆酶产量和生物质脱木质素
嫩椰子纤维是一种丰富的农业废弃物,由于其复杂的木质纤维素组成,需要化学或生物预处理以提高其增值应用的潜力。用产生漆酶的白腐真菌预处理嫩椰子纤维是一种有效的脱木质素和废物管理的可持续策略。本研究利用白腐菌Pleurotus pulmonarius对嫩椰子纤维进行预处理,通过固态发酵提高漆酶产量,实现去木素化。本研究通过响应面法(response surface methodology, RSM)探索不同因素对漆酶产率的影响,了解各因素之间的关系,以获得最大的漆酶产率。采用中心组合设计优化工艺参数,包括pH、温度、培养时间和玉米浸泡液浓度。优化后的工艺参数为pH为5.6,温度为27℃,培养时间为21 d, CSL浓度为3%。优化后的酶活性显著提高,达到663.76±10.67 IU/ml,优化后的酶活性提高了13.16倍。采用扫描电镜(SEM)和红外光谱(FTIR)分析观察了原纤维和去木素纤维表面结构的变化。增强的漆酶活性表明,嫩椰子纤维有潜力用于生产漆酶,这是以前从未探索过的。这种方法为管理柔软的椰子纤维提供了一种解决方案,并且由于改性纤维的性能增强,释放了增值的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biomass Conversion and Biorefinery
Biomass Conversion and Biorefinery Energy-Renewable Energy, Sustainability and the Environment
CiteScore
7.00
自引率
15.00%
发文量
1358
期刊介绍: Biomass Conversion and Biorefinery presents articles and information on research, development and applications in thermo-chemical conversion; physico-chemical conversion and bio-chemical conversion, including all necessary steps for the provision and preparation of the biomass as well as all possible downstream processing steps for the environmentally sound and economically viable provision of energy and chemical products.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信