Synthesis and characterization of Li and Zn doped bioactive glass_ ceramics for application in bone tissue engineering

IF 2.3 4区材料科学 Q2 MATERIALS SCIENCE, CERAMICS

Journal of Sol-Gel Science and Technology Pub Date : 2025-04-02 DOI:10.1007/s10971-025-06716-6

Arman Sedghi, Fatemeh Sobhan, Amirhossein Moghanian, Fatemeh Shahriyari, Soolmaz Heidari

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引用次数: 0

Abstract

In this research, the effects of co-additions of 5 mol% Li₂O and various amounts of ZnO (2–10 mol%) on the structure and biological properties of bioactive glass 58S were investigated. The synthesized glass-ceramics via sol-gel method were evaluated using X-ray diffraction technique, Fourier-transform infrared spectroscopy (FTIR) and thermal gravimetric/differential thermal analysis (TG/DTA) techniques. Then, the samples were placed in the simulated body fluid (SBF) for various durations to investigate the biological properties. Inductively coupled plasma optical emission spectroscopy (ICP-OES) and field-emission scanning electron microscopy (FESEM) equipped with energy-dispersive X-ray spectroscopy (EDX or EDS) were used to evaluate the ion release and the formation of calcium phosphate precipitates after SBF immersion. MTT assay and alkaline phosphatase activity examinations were applied to investigate the effect of glass-ceramics on the MG63 cells. Also, the antibacterial activities were examined using the agar plate test. The results showed that the Zn²⁺ and Li⁺ co-addition increased the degree of crystallinity while decreasing the crystallization temperature. Calcium phosphate precipitates were only detected in the bioactive glass-ceramic 58S group. However, the glass-ceramics containing 4% and 6% ZnO (both with 5% Li₂O) showed the best results in the MTT and ALP activity assays. The highest antibacterial activity was related to the bioactive glass-ceramic 58S. The addition of ZnO and Li₂O in appropriate concentrations can positively affect cell viability and alkaline phosphatase activity.

Graphical Abstract

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用于骨组织工程的Li和Zn掺杂生物活性玻璃陶瓷的合成与表征

本研究考察了5 mol% Li2O和不同氧化锌（2-10 mol%）共添加对生物活性玻璃58S结构和生物学性能的影响。采用x射线衍射技术、傅里叶变换红外光谱（FTIR）和热重/差热分析（TG/DTA）技术对溶胶-凝胶法制备的微晶玻璃进行了评价。然后，将样品放置在模拟体液（SBF）中不同时间，以研究其生物学特性。采用电感耦合等离子体光学发射光谱（ICP-OES）和场发射扫描电镜（FESEM），配以能量色散x射线能谱（EDX或EDS），对SBF浸泡后的离子释放和磷酸钙沉淀的形成进行了评价。采用MTT法和碱性磷酸酶活性法研究微晶玻璃对MG63细胞的影响。并用琼脂平板法检测其抑菌活性。结果表明：Zn2+和Li+的共加入提高了结晶度，降低了结晶温度；仅在生物活性玻璃陶瓷58S组检测到磷酸钙沉淀。然而，含有4%和6% ZnO（均含有5% Li2O）的微晶玻璃在MTT和ALP活性测试中表现出最好的结果。抗菌活性最高的是具有生物活性的58S玻璃陶瓷。适量添加ZnO和Li2O对细胞活力和碱性磷酸酶活性有积极影响。图形抽象

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来源期刊

Journal of Sol-Gel Science and Technology 工程技术-材料科学：硅酸盐

CiteScore

4.70

自引率

4.00%

发文量

280

审稿时长

2.1 months

期刊介绍： The primary objective of the Journal of Sol-Gel Science and Technology (JSST), the official journal of the International Sol-Gel Society, is to provide an international forum for the dissemination of scientific, technological, and general knowledge about materials processed by chemical nanotechnologies known as the "sol-gel" process. The materials of interest include gels, gel-derived glasses, ceramics in form of nano- and micro-powders, bulk, fibres, thin films and coatings as well as more recent materials such as hybrid organic-inorganic materials and composites. Such materials exhibit a wide range of optical, electronic, magnetic, chemical, environmental, and biomedical properties and functionalities. Methods for producing sol-gel-derived materials and the industrial uses of these materials are also of great interest.