Spatial metabolomics boost understanding the molecular targets and pathways of active components of traditional Chinese medicine in situ and in vivo

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-05-13 DOI:10.1016/j.trac.2025.118288

Kunshuang Shen , Le Yang , Hui Sun , Ye Sun , Guangli Yan , Ying Han , Xijun Wang

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Abstract

Traditional Chinese medicine (TCM), despite its therapeutic potential, faces challenges in mechanistic validation due to phytochemical complexity. Recent advances in spatial metabolomics, leveraging high-resolution mass spectrometry imaging (MSI), have provided exceptional spatial resolution and molecular specificity. This label-free technique enables the in situ spatial mapping of molecular production and localization, providing profound insights into the metabolic heterogeneity and mechanisms of TCM's active components. This review comprehensively explores the applications of spatial metabolomics in TCM research, focusing on biosynthesis, spatial distribution, pharmacodynamic mechanisms, safety evaluations, and pharmacokinetics. It also underscores the technological advancements and methodological improvements in spatial metabolomics in terms of sensitivity, spatial resolution, multimodal integration, and artificial intelligence (AI)-driven analytics. These applications reveal the spatial distribution and molecular targets of bioactive components, fostering TCM's broader acceptance in global healthcare systems.

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空间代谢组学有助于了解中药活性成分在体内和体内的分子靶点和途径

尽管中药具有治疗潜力，但由于植物化学的复杂性，其机制验证面临挑战。空间代谢组学的最新进展，利用高分辨率质谱成像（MSI），提供了卓越的空间分辨率和分子特异性。这种无标记技术使分子生成和定位的原位空间定位成为可能，为了解中药活性成分的代谢异质性和机制提供了深刻的见解。本文从生物合成、空间分布、药效学机制、安全性评价和药代动力学等方面全面探讨了空间代谢组学在中药研究中的应用。它还强调了空间代谢组学在灵敏度、空间分辨率、多模式集成和人工智能（AI）驱动分析方面的技术进步和方法改进。这些应用揭示了生物活性成分的空间分布和分子靶点，促进了中医药在全球医疗保健系统中的广泛接受。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.