New insights on the Rickettsia-tick-wild bird associations in the Western Amazon, Brazil

Abstract

Several studies have highlighted the importance of balancing wildlife and plant life to mitigate the spread of ticks and their pathogenic microorganisms. This balance is essential for preserving the health of wildlife, domestic animals, and, consequently, human populations. However, human activities, characterized by habitat destruction and fragmentation, have brought humans and domestic animals closer to wild bird populations, which act as dispersers of ticks of public health importance. In light of these challenges, this study aimed to determine the frequency of occurrence and abundance of tick species in wild birds in the upland forests of the southwestern Brazilian Amazon. Data were collected in two municipalities: Rio Branco (four urban forest fragments) and Manoel Urbano (an area of continuous forest in the Parque Estadual Chandless), both located in the state of Acre, Brazil. Birds were captured between May and September 2021 using 10 mist nets placed in five transects of each study area, resulting in a sampling effort of 1210 net hours. Captured birds were thoroughly inspected for tick infestation, and when found, they were removed using forceps. These ticks were identified using a stereomicroscope and specific taxonomic keys, as well as molecular analysis by PCR. We captured 315 birds, of which 95 (30.15 %) were infested with ticks in the larval and nymph stages. A total of 368 ticks belonging to the genus Amblyomma were collected. This included species such as Amblyomma coelebs, Amblyomma geayi, Amblyomma humerale, Amblyomma longirostre, Amblyomma nodosum, and Amblyomma maculatum sensu lato (s.l.). These results highlight 13 new associations between wild birds and ticks of the genus Amblyomma and reveal, for the first time, the presence of Amblyomma maculatum s.l. and rickettsiae from the Spotted Fever Group. These findings expand the knowledge of the epidemiology of ticks and rickettsial agents of public health importance in the region.