A bimetallic nanozyme with high peroxidase-like activity for visual detection of organophosphorus pesticides

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-05-16 DOI:10.1016/j.talanta.2025.128309

Jia Liu, Xin-Yue Chen, Juan Chen

{"title":"A bimetallic nanozyme with high peroxidase-like activity for visual detection of organophosphorus pesticides","authors":"Jia Liu, Xin-Yue Chen, Juan Chen","doi":"10.1016/j.talanta.2025.128309","DOIUrl":null,"url":null,"abstract":"<div><div>The detrimental effects of organophosphorus pesticides residues in food on human health demonstrate the crucial need for advanced detection method. To overcome the limitations of existing techniques, this study introduces a novel colorimetric approach combined with nanozyme technology for the detection of organophosphorus pesticides residues. Specifically, a bimetallic metal-organic framework (MOF) incorporating Co (II) and Mn (II) as metal nodes was developed, exhibiting enhanced synergistic properties. This Co/Mn-MOFs serves as a highly efficient peroxidase-like (POD-like) nanozyme, enabling the construction of a simple, highly sensitive colorimetric detection platform. The platform demonstrates exceptional performance in the “switch-on” detection of chlorpyrifos (CPF), achieving a linear detection range of 10–200 nM and a limit of detection (LOD) of 9.84 nM. To further enhance practicality, the platform was adapted into a portable test kit comprising a test swab and a processing tube. When equipped with a color-reading mobile application, the kit achieves an impressive LOD of 12.59 nM for CPF detection. The results highlight the robustness and reliability of this colorimetric method, validating its effectiveness for real-world applications. This work not only presents an innovative strategy for utilizing bimetallic MOFs as highly efficient POD-like nanozymes, but also showcases their exceptional potential in the sensitive and practical detection of organophosphorus pesticides.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"295 ","pages":"Article 128309"},"PeriodicalIF":6.1000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039914025007994","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The detrimental effects of organophosphorus pesticides residues in food on human health demonstrate the crucial need for advanced detection method. To overcome the limitations of existing techniques, this study introduces a novel colorimetric approach combined with nanozyme technology for the detection of organophosphorus pesticides residues. Specifically, a bimetallic metal-organic framework (MOF) incorporating Co (II) and Mn (II) as metal nodes was developed, exhibiting enhanced synergistic properties. This Co/Mn-MOFs serves as a highly efficient peroxidase-like (POD-like) nanozyme, enabling the construction of a simple, highly sensitive colorimetric detection platform. The platform demonstrates exceptional performance in the “switch-on” detection of chlorpyrifos (CPF), achieving a linear detection range of 10–200 nM and a limit of detection (LOD) of 9.84 nM. To further enhance practicality, the platform was adapted into a portable test kit comprising a test swab and a processing tube. When equipped with a color-reading mobile application, the kit achieves an impressive LOD of 12.59 nM for CPF detection. The results highlight the robustness and reliability of this colorimetric method, validating its effectiveness for real-world applications. This work not only presents an innovative strategy for utilizing bimetallic MOFs as highly efficient POD-like nanozymes, but also showcases their exceptional potential in the sensitive and practical detection of organophosphorus pesticides.

Abstract Image

查看原文本刊更多论文

一种具有高过氧化物酶样活性的双金属纳米酶用于有机磷农药的视觉检测

食品中有机磷农药残留对人体健康的危害表明，迫切需要先进的检测方法。为了克服现有技术的局限性，本研究提出了一种结合纳米酶技术的新型比色法检测有机磷农药残留的方法。具体来说，以Co （II）和Mn （II）为金属节点的双金属金属有机骨架（MOF）被开发出来，表现出增强的协同性能。这种Co/ mn - mof作为一种高效的过氧化物酶（POD-like）纳米酶，可以构建一个简单、高灵敏度的比色检测平台。该平台在毒死蜱（CPF）的“开关”检测方面表现出色，线性检测范围为10-200 nM，检出限（LOD）为9.84 nM。为了进一步提高实用性，该平台被改编为便携式测试套件，包括测试拭子和处理管。当配备颜色读取移动应用程序时，该试剂盒实现了令人印象深刻的12.59 nM的CPF检测LOD。结果突出了该比色法的鲁棒性和可靠性，验证了其在实际应用中的有效性。这项工作不仅提出了利用双金属mof作为高效pod类纳米酶的创新策略，而且还展示了它们在灵敏和实用的有机磷农药检测方面的特殊潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.