The miR-146a-associated HDAC2 regulation of PI3K is involved in pancreatitis in vitro

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-05-20 DOI:10.1016/j.bbrep.2025.102057

Ding-wen Zhong , Xiang-tian Zeng , Wen-hui Chen , Xian-yu Huang , Jia-xin Liu , Yong-hui Liao

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引用次数: 0

Abstract

Purpose

To investigate the association between miR-146a/HDAC2 and their regulatory roles on the PI3K expression during pancreatitis.

Methods

Rat pancreatic AR42J cells were treated with LPS for simulating pancreatitis. Expression levels of inflammatory factors (IL-6, TNF-α) and miR-146a were detected to determine the optimal LPS concentration for establishing an in vitro pancreatitis model. Cell proliferation and apoptosis were analyzed using CCK-8 and flow cytometry. Immunofluorescence was performed to assess co-localization of HDAC2 and PI3K. ELISA quantified TNF-α and IL-6 levels in cell supernatants. A dual-luciferase assay verified the targeting relationship between miR-146a and HDAC2.

Results

Compared to controls, the cell proliferation ability of the pancreatitis model group was decreased, whereas TSA and miR-146a mimic interventions restored proliferation. The expression of IL-6 and TNF-αin the LPS group was higher than that in the control group, and their expression decreases in the TSA and miR-146a mimic intervention group. Besides the dual luciferase detected the targeting relationship between miR-146a and HDAC2, the immunofluorescence showed co-localization of HDAC2 and PI3K.

Conclusions

TSA and miR-146a mimic enhance proliferation and reduce inflammation in pancreatitis cells. The miR-146a/HDAC2 axis may mediate therapeutic effects in pancreatitis by modulating the PI3K expression.

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mir -146a相关的HDAC2对PI3K的调控参与体外胰腺炎

目的探讨miR-146a/HDAC2对胰腺炎患者PI3K表达的调控作用。方法采用LPS对大鼠胰腺AR42J细胞进行模拟胰腺炎处理。检测炎症因子（IL-6、TNF-α）和miR-146a的表达水平，确定建立体外胰腺炎模型的最佳LPS浓度。CCK-8和流式细胞术分析细胞增殖和凋亡情况。免疫荧光法评估HDAC2和PI3K的共定位。ELISA测定细胞上清液中TNF-α和IL-6水平。双荧光素酶测定证实了miR-146a和HDAC2之间的靶向关系。结果与对照组相比，胰腺炎模型组细胞增殖能力下降，而TSA和miR-146a模拟干预恢复了细胞增殖。LPS组IL-6、TNF-α表达高于对照组，TSA和miR-146a模拟干预组IL-6、TNF-α表达降低。除了双荧光素酶检测miR-146a与HDAC2的靶向关系外，免疫荧光显示HDAC2与PI3K共定位。结论stsa和miR-146a可促进胰腺炎细胞增殖，减轻炎症反应。miR-146a/HDAC2轴可能通过调节PI3K表达介导胰腺炎的治疗作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.