Yongkang Liu, Yang Zhu, Sijing Wan, Xianzhong Wang, Liwen Guan, Caidi Xu, Binghua Xie, Shigui Wang, Sisi Sun, Bin Tang
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引用次数: 0
Abstract
Trehalase (TRE) is an important enzyme that is responsible for trehalose hydrolysis. However, the effect of NLTRE on the reproduction of Nilaparvata lugens has not been clearly reported. To comprehensively evaluate the pest control potential of NLTRE, this study analyzed the effect of NLTRE on female reproduction of N. lugens by inhibiting TRE with dsTREs injection at mRNA level and validamycin injection at protein level, respectively. The results showed that validamycin not only significantly reduced the female body weight, but also extended the preoviposition time, but dsTREs had no significant effect on these phenotypes. Besides, validamycin significantly inhibited the ovarian development of females in the early stage, while dsTREs affected the ovarian development in the later stage. However, both two treatments have extremely significantly reduced the total number of eggs laid by female, and the egg hatchability also was extremely significantly decreased, likely due to the destruction of chitin components in egg shells. Therefore, TRE inhibition can decrease the fecundity of N. lugens female, which suggest that TRE is a potential pest control target.
期刊介绍:
Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues.
Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates.
Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management.
Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.