Trehalase regulates ovarian maturation and egg hatchability of Nilaparvata lugens

IF 4.3 1区农林科学 Q1 ENTOMOLOGY

Journal of Pest Science Pub Date : 2025-05-19 DOI:10.1007/s10340-025-01903-4

Yongkang Liu, Yang Zhu, Sijing Wan, Xianzhong Wang, Liwen Guan, Caidi Xu, Binghua Xie, Shigui Wang, Sisi Sun, Bin Tang

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Abstract

Trehalase (TRE) is an important enzyme that is responsible for trehalose hydrolysis. However, the effect of NLTRE on the reproduction of Nilaparvata lugens has not been clearly reported. To comprehensively evaluate the pest control potential of NLTRE, this study analyzed the effect of NLTRE on female reproduction of N. lugens by inhibiting TRE with dsTREs injection at mRNA level and validamycin injection at protein level, respectively. The results showed that validamycin not only significantly reduced the female body weight, but also extended the preoviposition time, but dsTREs had no significant effect on these phenotypes. Besides, validamycin significantly inhibited the ovarian development of females in the early stage, while dsTREs affected the ovarian development in the later stage. However, both two treatments have extremely significantly reduced the total number of eggs laid by female, and the egg hatchability also was extremely significantly decreased, likely due to the destruction of chitin components in egg shells. Therefore, TRE inhibition can decrease the fecundity of N. lugens female, which suggest that TRE is a potential pest control target.

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海藻糖酶调控褐飞虱卵巢成熟和卵孵化率

海藻糖酶（TRE）是一种重要的海藻糖水解酶。然而，NLTRE对褐飞虱繁殖的影响尚未见明确报道。为了综合评价NLTRE的防虫潜力，本研究通过注射dstress在mRNA水平上抑制NLTRE，注射validamycin在蛋白水平上抑制NLTRE，分析了NLTRE对褐家蝇雌虫繁殖的影响。结果表明，缬霉素不仅显著降低了雌性体重，而且延长了产卵前时间，但dsTREs对这些表型没有显著影响。此外，validamycin在早期显著抑制雌性卵巢发育，而dstress在后期影响卵巢发育。然而，这两种处理都极显著地降低了雌虫的产卵总数，卵的孵化率也极显著地降低，这可能是由于蛋壳中的几丁质成分被破坏所致。因此，抑制TRE可降低褐飞虱雌虫的繁殖力，表明TRE是潜在的害虫防治靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pest Science 生物-昆虫学

CiteScore

10.40

自引率

8.30%

发文量

114

审稿时长

6-12 weeks

期刊介绍： Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.