A bioprocess optimization study to enhance the production of Menaquinone-7 using Bacillus subtilis MM26.

IF 4.3 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbial Cell Factories Pub Date : 2025-05-16 DOI:10.1186/s12934-025-02735-8

Maneesha M, Subathra Devi C

{"title":"A bioprocess optimization study to enhance the production of Menaquinone-7 using Bacillus subtilis MM26.","authors":"Maneesha M, Subathra Devi C","doi":"10.1186/s12934-025-02735-8","DOIUrl":null,"url":null,"abstract":"Background: Menaquinone-7 (MK-7) has a vital significance in promoting human health and tackling several global health concerns which makes its production extremely important. MK-7 is not easily accessible at a reasonable cost due to the poor fermentation yields and the existence of several laborious downstream unit processes. Efficient manufacturing methods are essential to meet the global requirements due to the increasing demand in the pharmaceutical and nutraceutical industries. This research study focuses on the enhanced production of MK-7 from Bacillus subtilis MM26 isolated from fermented home-made wine.Results: A suitable MK-7 production medium for Bacillus subtilis MM26 was determined and the yield was found to be 67 ± 0.6 mg/L. The one factor at a time (OFAT) results showed that medium containing lactose, glycine, with a pH 7, a temperature of 37 °C, and an inoculum size of 2.5% (2 × 10⁶ CFU/mL) was optimal synthesis of MK-7. RSM indicated that incubation time, carbon and nitrogen sources were the factors significantly affecting the MK-7 yield. RSM predicted optimal conditions, which yielded a maximum concentration of 442 ± 2.08 mg/L of MK-7.Conclusions: The outcomes of this study demonstrated the potential of Bacillus subtilis MM26 in large-scale industrial production of MK-7. The yield of MK-7 was amplified efficiently by integration of OFAT and RSM, paving the way for cost-efficient industrial production.","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"109"},"PeriodicalIF":4.3000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082928/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial Cell Factories","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s12934-025-02735-8","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Menaquinone-7 (MK-7) has a vital significance in promoting human health and tackling several global health concerns which makes its production extremely important. MK-7 is not easily accessible at a reasonable cost due to the poor fermentation yields and the existence of several laborious downstream unit processes. Efficient manufacturing methods are essential to meet the global requirements due to the increasing demand in the pharmaceutical and nutraceutical industries. This research study focuses on the enhanced production of MK-7 from Bacillus subtilis MM26 isolated from fermented home-made wine.

Results: A suitable MK-7 production medium for Bacillus subtilis MM26 was determined and the yield was found to be 67 ± 0.6 mg/L. The one factor at a time (OFAT) results showed that medium containing lactose, glycine, with a pH 7, a temperature of 37 °C, and an inoculum size of 2.5% (2 × 10⁶ CFU/mL) was optimal synthesis of MK-7. RSM indicated that incubation time, carbon and nitrogen sources were the factors significantly affecting the MK-7 yield. RSM predicted optimal conditions, which yielded a maximum concentration of 442 ± 2.08 mg/L of MK-7.

Conclusions: The outcomes of this study demonstrated the potential of Bacillus subtilis MM26 in large-scale industrial production of MK-7. The yield of MK-7 was amplified efficiently by integration of OFAT and RSM, paving the way for cost-efficient industrial production.

查看原文本刊更多论文

枯草芽孢杆菌MM26提高甲基萘醌-7产量的生物工艺优化研究。

背景：甲基萘醌-7 （MK-7）在促进人类健康和解决几个全球健康问题方面具有重要意义，因此其生产极为重要。由于发酵产量低和存在几个费力的下游单元过程，MK-7不容易以合理的成本获得。由于制药和营养保健行业的需求不断增加，高效的制造方法对于满足全球需求至关重要。本研究以自制发酵酒中分离的枯草芽孢杆菌MM26为原料，对MK-7进行了强化生产。结果：确定了适合枯草芽孢杆菌MM26产MK-7的培养基，产率为67±0.6 mg/L。OFAT实验结果表明，含乳糖、甘氨酸、pH为7、温度为37℃、接种量为2.5% （2 × 10⁶CFU/mL）的培养基是MK-7的最佳合成条件。RSM结果表明，培养时间、碳源和氮源是影响MK-7产量的主要因素。RSM预测的最佳条件为MK-7的最大浓度为442±2.08 mg/L。结论：本研究结果表明枯草芽孢杆菌MM26具有大规模工业化生产MK-7的潜力。通过OFAT和RSM的结合，有效地提高了MK-7的产量，为具有成本效益的工业生产铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microbial Cell Factories 工程技术-生物工程与应用微生物

CiteScore

9.30

自引率

4.70%

发文量

235

审稿时长

2.3 months

期刊介绍： Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems